HLA-DR1 antibody (PE)

Catalog No. ABIN302033

The Mouse Monoclonal anti-HLA-DR1 antibody has been validated for FACS. It is suitable to detect HLA-DR1 in samples from Human.

Quick Overview for HLA-DR1 antibody (PE) (ABIN302033)

Target: HLA-DR1 (MHC Class II HLA-DR1 (HLA-DR1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HLA-DR1 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Clone: MEM-267

Product Details anti-HLA-DR1 Antibody (PE)

Purpose: Anti-HLA-DR1 (empty) PE

Specificity: The antibody MEM-267 specifically binds extracellularly to the empty but not peptide-loaded form of HLA-DR1. DR is the isotypes of human MHC Class II molecules expressed on antigen-presenting cells (APC, dendritic cells, B lymphocytes, monocytes, macrophages).

Cross-Reactivity (Details): Human

Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: Purified, insoluble DR1 beta chain (DRB1*0101) expressed in E. coli inclusion bodies.

Isotype: IgG2b

Application Details

Application Notes: Flow cytometry: The antibody MEM-267 stains immature dendritic cells that express empty cell surface MHC molecules, but not cells that express predominantly peptide loaded forms. Recommended dilution: 1-5 μg/mL.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.
Avoid prolonged exposure to light.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Target Details for HLA-DR1

Target: HLA-DR1 (MHC Class II HLA-DR1 (HLA-DR1))

Alternative Name: HLA-DR1

Background: HLA-DR1 belongs to the HLA class II beta chain paralogues. The MHC Class II molecule is a heterodimer consisting of an alpha (DRA) and a beta chain (DRB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. MHC Class II molecules are expressed in antigen presenting cells (APC). The beta chain is approximately 26-28 kDa. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Hundreds of DRB1 alleles have been described and typing for these polymorphisms is routinely done for bone marrow and kidney transplantation.