TUBG1 antibody (AA 434-449)

Catalog No. ABIN302067

This anti-TUBG1 antibody is a Mouse Monoclonal antibody detecting TUBG1 in WB and ICC. Suitable for Plant, Protozoa and Mammalian. This Primary Antibody has been cited in 11+ publications.

Quick Overview for TUBG1 antibody (AA 434-449) (ABIN302067)

Target: TUBG1 (Tubulin, gamma 1 (TUBG1))

Reactivity: Plant, Protozoa, Mammalian

Host: Mouse

Clonality: Monoclonal

Conjugate: This TUBG1 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC)

Clone: TU-32

Product Details anti-TUBG1 Antibody

Binding Specificity: AA 434-449

Purpose: Anti-gamma-Tubulin Purified

Specificity: The antibody TU-32 recognizes C-terminus (amino acids 434-449 in human) of gamma-tubulin, a 48 kDa structural constituent of cytoskeleton and microtubule organizing center (MTOC). The epitope was located in the aminoacid sequence PDYISW (aa441-446 in human), which is identical for gamma-tubulin 1 and gamma-tubulin 2.

Cross-Reactivity (Details): Animals, Protozoa, Plants

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: human gamma-tubulin peptide EYHAATRPDYISWGTQ, amino acids 434-449

Isotype: IgG1

Application Details

Application Notes: Western blotting: Recommended dilution: 1-2 μg/mL, reducing conditions.
Immunocytochemistry: Methanol/acetone fixation required.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-TUBG1 antibody (ABIN302067)

Katsetos, Dráberová, Smejkalová, Reddy, Bertrand, de Chadarévian, Legido, Nissanov, Baas, Dráber: "Class III beta-tubulin and gamma-tubulin are co-expressed and form complexes in human glioblastoma cells." in: Neurochemical research, Vol. 32, Issue 8, pp. 1387-98, (2007) (PubMed).

Katsetos, Reddy, Dráberová, Smejkalová, Del Valle, Ashraf, Tadevosyan, Yelin, Maraziotis, Mishra, Mörk, Legido, Nissanov, Baas, de Chadarévian, Dráber: "Altered cellular distribution and subcellular sorting of gamma-tubulin in diffuse astrocytic gliomas and human glioblastoma cell lines." in: Journal of neuropathology and experimental neurology, Vol. 65, Issue 5, pp. 465-77, (2006) (PubMed).

Sulimenko, Dráberová, Sulimenko, Macurek, Richterová, Dráber, Dráber: "Regulation of microtubule formation in activated mast cells by complexes of gamma-tubulin with Fyn and Syk kinases." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 176, Issue 12, pp. 7243-53, (2006) (PubMed).

Libusová, Sulimenko, Sulimenko, Hozák, Dráber: "gamma-Tubulin in Leishmania: cell cycle-dependent changes in subcellular localization and heterogeneity of its isoforms." in: Experimental cell research, Vol. 295, Issue 2, pp. 375-86, (2004) (PubMed).

Linhartová, Novotná, Sulimenko, Dráberová, Dráber: "Gamma-tubulin in chicken erythrocytes: changes in localization during cell differentiation and characterization of cytoplasmic complexes." in: Developmental dynamics : an official publication of the American Association of Anatomists, Vol. 223, Issue 2, pp. 229-40, (2002) (PubMed).

Sulimenko, Sulimenko, Poznanovic, Nechiporuk-Zloy, Böhm, Macurek, Unger, Dráber: "Association of brain gamma-tubulins with alpha beta-tubulin dimers." in: The Biochemical journal, Vol. 365, Issue Pt 3, pp. 889-95, (2002) (PubMed).

Dráberová, Mac?rek, Richterová, Böhm, Dráber: "Monoclonal antibody KN-01 against the heavy chain of kinesin." in: Folia biologica, Vol. 48, Issue 2, pp. 77-9, (2002) (PubMed).

Nohýnkova, Dráber, Reischig, Kulda: "Localization of gamma-tubulin in interphase and mitotic cells of a unicellular eukaryote, Giardia intestinalis." in: European journal of cell biology, Vol. 79, Issue 6, pp. 438-45, (2000) (PubMed).

Binarová, Cenklová, Hause, Kubátová, Lysák, Dolezel, Bögre, Dráber: "Nuclear gamma-tubulin during acentriolar plant mitosis." in: The Plant cell, Vol. 12, Issue 3, pp. 433-42, (2000) (PubMed).

Dráberová, Dráberová, Surviladze, Dráber, Dráber: "Protein tyrosine kinase p53/p56(lyn) forms complexes with gamma-tubulin in rat basophilic leukemia cells." in: International immunology, Vol. 11, Issue 11, pp. 1829-39, (1999) (PubMed).

Nováková, Dráberová, Schürmann, Czihak, Viklický, Dr-aber: "gamma-Tubulin redistribution in taxol-treated mitotic cells probed by monoclonal antibodies." in: Cell motility and the cytoskeleton, Vol. 33, Issue 1, pp. 38-51, (1996) (PubMed).

Target Details for TUBG1

Target: TUBG1 (Tubulin, gamma 1 (TUBG1))

Alternative Name: gamma-Tubulin

Background: Tubulin gamma 1,The gamma-tubulin (TUBG1, relative molecular weight about 48 kDa) is a minor member of tubulin family (less that 0.01 % of tubulin dimer). The gamma-tubulin ring structures, however, serve to provide structural primer for initiation of microtubular nucleation and growth, thereby being crutial for microtubule-based cellular processes, above all for mitotic spindle formation. In animal cells, a center of microtubule organization is the centrosome composed of a pair of cylindrical centrioles surrounded by fibrous pericentriolar material containing gamma-tubulin. Formation of the mitotic spindle is preceded by duplication of centrosome during S phase. Before mitosis, both centrosomes increase their microtubule nucleation capacity and form two microtuble asters that are pushed apart from each other by the forces of motor proteins associated at the microtubule surface. Humans possess two gamma-tubulin genes. Gamma-tubulin 1 represents a ubiquitous isotype, whereas gamma-tubulin 2 is found predominantly in the brain, where it may be endowed with divergent functions beyond microtubule nucleation.,TUBG

Gene ID: 7283

UniProt: P23258

Pathways: Microtubule Dynamics, M Phase