Sos antibody

Catalog No. ABIN302096

The Mouse Monoclonal anti- antibody has been validated for WB and ICC. It is suitable to detect in samples from Human, Mouse and Non-Human Primate.

Quick Overview for Sos antibody (ABIN302096)

Target: Sos

Reactivity: Human, Mouse, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC)

Clone: SOS-01

Product Details

Purpose: Anti-SOS Purified

Specificity: The antibody SOS-01 reacts with human Sos, an ubiquitously expressed 150 kDa intracellular protein. The epitope sequence is highly conserved and reactivity with multiple species is expected.

Cross-Reactivity (Details): Human, Mouse, Other not determined

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Peptide corresponding to amino acids THPSMHRDGPPLLENAHSS of human Sos protein.

Isotype: IgG1

Application Details

Application Notes: Western blotting: Recommended dilution: 1 μg/mL, positive control: HeLa human cervix carcinoma cell line, reducing conditions.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

Target Details

Target: Sos

Background: SOS Ras/Rac guanine nucleotide exchange factor 1,The guanine nucleotide exchange factor Sos (son-of-sevenless) is a complex multidomain protein that activates the small GTPase Ras (H-Ras, K-Ras, N-Ras, but not functionally distinct R-Ras) in response to receptor tyrosine kinase stimulation. Nucleotide exchange activity of Sos is stimulated by allosteric Ras binding. By another (separable) guanine exchange factor domain domain Sos modulates activity of Rac/Rho GTPases. Sos thus integrates signals that affect both gene expression and cytoskeletal reorganization, the Sos-mediated Ras-activation and Rac activation differ in composition and stability of the formed complex.,SOS1, HGF, GGF1

Gene ID: 6654

UniProt: Q07889