Lactate Dehydrogenase C antibody (AA 217-231)

Catalog No. ABIN302132

The Goat Polyclonal anti-Lactate Dehydrogenase C antibody has been validated for WB, ELISA and IHC. It is suitable to detect Lactate Dehydrogenase C in samples from Mouse.

Quick Overview for Lactate Dehydrogenase C antibody (AA 217-231) (ABIN302132)

Target: Lactate Dehydrogenase C (LDHC)

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This Lactate Dehydrogenase C antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-Lactate Dehydrogenase C Antibody

Binding Specificity: AA 217-231

Purpose: LDHC (aa 217 - 231)

Sequence: KLGTDSDKEH WKNIH

Specificity: Both variants represent identical product (NP_002292.1 and NP_059144.1).

Cross-Reactivity: Human, Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-KLGTDSDKEHWKNIH

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Mouse Testis shows strong signal in seminiferous tubules of Mouse Tesits. Recommended concentration, 1-2 μg/mL. This antibody has been successfully used in IHC on Mouse, PMID: 36464740.

Western Blot: Approx 30-35 kDa band observed in Mouse Testis lysates (calculated MW of 36.6 kDa according to human NP_002292.1 and 35.9 kDa according to mouse NP_038608.1). Recommended concentration: 0.03-0.1 μg/mL. This antibody has been successfully used

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Lactate Dehydrogenase C

Target: Lactate Dehydrogenase C (LDHC)

Alternative Name: LDHC

Background: LDHC, MGC111073, lactate dehydrogenase C, LDH3, LDHX

Gene ID: 3948, 16833

NCBI Accession: NP_002292, NP_059144

Pathways: Ribonucleoside Biosynthetic Process, Warburg Effect