ACTN1 antibody (AA 1-330)

Catalog No. ABIN3021587

This anti-ACTN1 antibody is a Rabbit Polyclonal antibody detecting ACTN1 in WB and IHC. Suitable for Human. This Primary Antibody has been cited in 3+ publications.

Quick Overview for ACTN1 antibody (AA 1-330) (ABIN3021587)

Target: ACTN1 (Actinin, alpha 1 (ACTN1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ACTN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-ACTN1 Antibody

Binding Specificity: AA 1-330

Sequence: MDHYDSQQTN DYMQPEEDWD RDLLLDPAWE KQQRKTFTAW CNSHLRKAGT QIENIEEDFR DGLKLMLLLE VISGERLAKP ERGKMRVHKI SNVNKALDFI ASKGVKLVSI GAEEIVDGNV KMTLGMIWTI ILRFAIQDIS VEETSAKEGL LLWCQRKTAP YKNVNIQNFH ISWKDGLGFC ALIHRHRPEL IDYGKLRKDD PLTNLNTAFD VAEKYLDIPK MLDAEDIVGT ARPDEKAIMT YVSSFYHAFS GAQKAETAAN RICKVLAVNQ ENEQLMEDYE KLASDLLEWI RRTIPWLENR VPENTMHAMQ QKLEDFRDYR RLHKPPKVQE

Cross-Reactivity: Human, Monkey, Mouse

Characteristics: Polyclonal Antibodies

Purification: Affinity purification

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-330 of human ACTN1 (NP_001123477.1).

Isotype: IgG

Application Details

Application Notes: WB,1:500 - 1:2000,IHC,1:50 - 1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze / thaw cycles

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

References for anti-ACTN1 antibody (ABIN3021587)

Zhang, Xu, Xu, Wei, Xu: "Protein kinase A inhibitor, H89, significantly enhances survival rate of dissociated human embryonic stem cells following cryopreservation." in: Cell proliferation, Vol. 49, Issue 5, pp. 589-98, (2017) (PubMed).

Zhang, Xu, Xu, Wei, Xu: "Protein kinase A inhibitor, H89, enhances survival and clonogenicity of dissociated human embryonic stem cells through Rho-associated coiled-coil containing protein kinase (ROCK) inhibition." in: Human reproduction (Oxford, England), Vol. 31, Issue 4, pp. 832-43, (2016) (PubMed).

Xu, Zhang, Xu, Wei, Xu: "Sensitivity of human embryonic stem cells to different conditions during cryopreservation." in: Cryobiology, Vol. 71, Issue 3, pp. 486-92, (2016) (PubMed).

Target Details for ACTN1

Target: ACTN1 (Actinin, alpha 1 (ACTN1))

Alternative Name: ACTN1

Background: Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene.,ACTN1,BDPLT15,Signal Transduction,Cell Biology & Developmental Biology,Apoptosis,Cytoskeleton,Microfilaments,Immunology & Inflammation,T Cell Receptor Signaling Pathway,Stem Cells,ACTN1

Molecular Weight: 102 kDa/103 kDa/105 kDa/107 kDa

Gene ID: 87

UniProt: P12814

Pathways: Cell-Cell Junction Organization