Complement Factor H antibody (AA 20-270)

Catalog No. ABIN3023097

The Rabbit Polyclonal anti-Complement Factor H antibody has been validated for WB, IHC and IF and has been independently validated. It is suitable to detect Complement Factor H in samples from Human. There is 1 publication available.

Quick Overview for Complement Factor H antibody (AA 20-270) (ABIN3023097)

Target: Complement Factor H (CFH)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Complement Factor H antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-Complement Factor H Antibody

Binding Specificity: AA 20-270

Sequence: DCNELPPRRN TEILTGSWSD QTYPEGTQAI YKCRPGYRSL GNVIMVCRKG EWVALNPLRK CQKRPCGHPG DTPFGTFTLT GGNVFEYGVK AVYTCNEGYQ LLGEINYREC DTDGWTNDIP ICEVVKCLPV TAPENGKIVS SAMEPDREYH FGQAVRFVCN SGYKIEGDEE MHCSDDGFWS KEKPKCVEIS CKSPDVINGS PISQKIIYKE NERFQYKCNM GYEYSERGDA VCTESGWRPL PSCEEKSCDN P

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Polyclonal Antibodies

Purification: Affinity purification

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 20-270 of human Complement factor H (NP_001014975.1).

Isotype: IgG

Application Details

Application Notes: WB,1:500 - 1:2000,IHC,1:50 - 1:200,IF,1:50 - 1:200

Restrictions: For Research Use only

Independent Validation (IF)

Validation #101403 (Immunofluorescence)

by: Alexander Lab, Jacobs School of Medicine and Biomedical Sciences, Department of Medicine, University of Buffalo

No.: #101403

Date: 07/19/2017

Antigen: Commplement Factor H

Lot Number: 4390101

Method validated: Immunofluorescence

Positive Control: MRL/lpr lupus mice (14 weeks of age)

Negative Control: FH knockout MRL/lpr lupus mice

Notes: Passed. The complement factor H antibody ABIN3023097 works very well on murine kidney samples and shows the expected CFH localization and strong staining.

Validation Images

IF staining with ABIN3023097 of kidneys harvested from 14 week old MRL/lpr mice sufficient and deficient in factor H. Cryosections were fixed, stained and images acquired as described in the protocol.

Full Methods

Primary Antibody: ABIN3023097

Secondary Antibody: goat anti-rabbit rhodamine conjugated antibody (ThermoFisher Scientific, T-2769, lot 6251)

Full Protocol:

• Prepare kidneys of wt and complement factor H ko MRL/lpr lupus mice of 14 weeks of age.
• Snap-freeze kidneys in 2-methylbutane cooled on dry ice and keep at −80°C until use.
• Sections were cut at 8µm using the cryostat.
• Dry sections for 30min at RT.
• Hydrate sections in PBS 2x for 15min at RT.
• Fix cryostat sections with 4% paraformaldehyde (PFA) for 15min at RT.
• Wash sections 2x with PBS for 15min at RT.
• Incubate sections with primary rabbit anti-Complement Factor H antibody (antibodies-online, ABIN3023097, 0004390101) diluted 1:100 in PBS ON at 4°C.
• Wash sections 2x for 15min with PBS.
• Incubate sections with secondary goat anti-rabbit rhodamine conjugated antibody (ThermoFisher Scientific, T-2769, lot 6251) diluted 1:100 in PBS for 30min at RT.
• Wash sections 2x for 15min with PBS.
• Incubate sections with ActinGreen 488 ReadyProbes Reagent (ThermoFisher Scientific, R37122, lot 1700569) diluted 1:100 in PBS for 30min at RT.
• Wash sections 2x for 15min with PBS.
• Image acquisition on a Zeiss microscope (Carl Zeiss, Oberkochen, Germany).

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze / thaw cycles

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

References for anti-Complement Factor H antibody (ABIN3023097)

Cheng, Malachi, Cipi, Ma, Brush, Agbaga, Punzo: "HK2 Mediated Glycolytic Metabolism in Mouse Photoreceptors Is Not Required to Cause Late Stage Age-Related Macular Degeneration-Like Pathologies." in: Biomolecules, Vol. 11, Issue 6, (2021) (PubMed).

Target Details for Complement Factor H

Target: Complement Factor H (CFH)

Alternative Name: CFH

Background: This gene is a member of the Regulator of Complement Activation (RCA) gene cluster and encodes a protein with twenty short consensus repeat (SCR) domains. This protein is secreted into the bloodstream and has an essential role in the regulation of complement activation, restricting this innate defense mechanism to microbial infections. Mutations in this gene have been associated with hemolytic-uremic syndrome (HUS) and chronic hypocomplementemic nephropathy. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.,CFH,AHUS1,AMBP1,ARMD4,ARMS1,CFHL3,FH,FHL1,HF,HF1,HF2,HUS,Immunology & Inflammation,Cell Intrinsic Innate Immunity Signaling Pathway,CFH

Molecular Weight: 51 kDa/139 kDa

Gene ID: 3075

UniProt: P08603

Pathways: Complement System, Cellular Response to Molecule of Bacterial Origin