Histone 3 antibody (H3K9me2)

Catalog No. ABIN3023260

This Rabbit Polyclonal antibody specifically detects Histone 3 in WB, IF, IHC, ChIP, IP and ChIP-seq. It exhibits reactivity toward Human and has been mentioned in 4+ publications.

Quick Overview for Histone 3 antibody (H3K9me2) (ABIN3023260)

Target: Histone 3 (H3) (Histone H3 (H3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Chromatin Immunoprecipitation (ChIP), Immunoprecipitation (IP), ChIP DNA-Sequencing (ChIP-seq)

Product Details anti-Histone 3 Antibody

Binding Specificity: H3K9me2

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Methylated Antibodies

Purification: Affinity purification

Immunogen: A synthetic methylated peptide corresponding to residues surrounding K9 of human histone H3

Isotype: IgG

Application Details

Application Notes: WB,1:500 - 1:2000,IHC,1:50 - 1:200,IF,1:50 - 1:200,IP,1:50 - 1:200,ChIP,1:50 - 1:200,ChIP-seq,1:50 - 1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze / thaw cycles

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

References for anti-Histone 3 antibody (ABIN3023260)

Zuo, Rong, Li, Lv, Lan, Tong: "The histone methyltransferase SETD2 is required for expression of acrosin-binding protein 1 and protamines and essential for spermiogenesis in mice." in: The Journal of biological chemistry, Vol. 293, Issue 24, pp. 9188-9197, (2019) (PubMed).

Vaughan, Dickson, Whelihan, Johnstone, Cornett, Cheek, Ausherman, Cowles, Sun, Rothbart: "Chromatin structure and its chemical modifications regulate the ubiquitin ligase substrate selectivity of UHRF1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 115, Issue 35, pp. 8775-8780, (2018) (PubMed).

Cao, Liu, Yue, Liu, Pei, Gu, Wang, Jia: "Iron chelation inhibits cancer cell growth and modulates global histone methylation status in colorectal cancer." in: Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine, Vol. 31, Issue 5, pp. 797-805, (2018) (PubMed).

Zhao, Lei, Zhang, Zheng, Wang, Zhao, Li, Ye, Li, Wei, Wu: "Global histone modification profiling reveals the epigenomic dynamics during malignant transformation in a four-stage breast cancer model." in: Clinical epigenetics, Vol. 8, pp. 34, (2016) (PubMed).

Target Details for Histone 3

Target: Histone 3 (H3) (Histone H3 (H3))

Alternative Name: Histone H3

Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails, instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.,H3.4,H3/g,H3FT,H3t,HIST3H3,Histone H3,HIST1H3A,Signal Transduction,MAPK-Erk Signaling Pathway,MAPK-P38 Signaling Pathway,Epigenetics & Nuclear Signaling,Epigenetic Modifications,Methylation,Histone H3

Molecular Weight: 15 kDa

Gene ID: 8290

UniProt: Q16695