pan Keratin antibody (PE)

Catalog No. ABIN3023996

This Mouse Monoclonal antibody specifically detects pan Keratin in IF and FACS. It exhibits reactivity toward Human, Rat, Mouse, Cow, Monkey, Dog, Rabbit and Chicken.

Quick Overview for pan Keratin antibody (PE) (ABIN3023996)

Target: pan Keratin (panKRT)

Reactivity: Human, Rat, Mouse, Cow, Monkey, Dog, Rabbit, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This pan Keratin antibody is conjugated to PE

Application: Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: AE1-AE3

Product Details anti-pan Keratin Antibody (PE)

Purity: Protein G affinity chromatography

Immunogen: Human epidermal keratin was used as the immunogen for this pan Cytokeratin antibody.

Isotype: IgG1

Application Details

Application Notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the pan Cytokeratin antibody AE1 + AE3 to be titered up or down for optimal performance.\. Flow Cytometry : 5 μL/test/million cells or 5 μL/test/100 μL of whole blood,Immunofluorescence : 1:50-1:100 for 30 minutes at RT (1)

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: 500 μL at 0.1 mg/mL with 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the pan Cytokeratin antibody at 2-8°C. Conjugate is light sensitive, store in the dark.

Target Details for pan Keratin

Target: pan Keratin (panKRT)

Alternative Name: Pan Cytokeratin

Background: Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI <5.7) and basic (pI >6.0) subfamilies. This pan keratin antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, which include CK1, CK3-6, CK8, CK10, CK14-16, and CK19. Many studies have shown the usefulness of cytokeratin markers in cancer research and tumor diagnosis. The AE1 + AE3 antibody cocktail is a broad spectrum pan cytokeratin antibody cocktail which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It has been used to characterize the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. It stains cytokeratin present in normal and abnormal human tissues and has shown high sensitivity in the recognition of epithelial cells and carcinomas.