beta Actin antibody (N-Term)

Catalog No. ABIN3024135

The Rabbit Polyclonal anti-beta Actin antibody has been validated for WB. It is suitable to detect beta Actin in samples from Human, Mouse, Rat, Chicken, Rabbit, Dog and Monkey.

Quick Overview for beta Actin antibody (N-Term) (ABIN3024135)

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Reactivity: Human, Mouse, Rat, Chicken, Rabbit, Dog, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This beta Actin antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-beta Actin Antibody

Binding Specificity: N-Term

Purification: Protein A Affinity Chromatography

Immunogen: A recombinant protein fragment from the N-terminus of human beta Actin was used as the immunogen for this antibody.

Isotype: IgG

Application Details

Application Notes: Provided assay concentrations are suggestions only, beta Actin antibody titration may be required for optimal results.\. Western blot: 1-3 μg/mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the loading control antibody and store frozen at -20°C or colder to avoid repeated freeze-thaw cycles.

Target Details for beta Actin

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Alternative Name: beta Actin

Background: Actins proteins are highly conserved and involved in cellular structure, integrity and motility. There are three main isoforms of actin in vertebrates: alpha, beta and gamma. Alpha actins are found in muscle cells while beta and gamma actins are non-muscle actins. Beta actin is commonly used as a loading control protein in western blot. An antibody to beta actin is used to demonstrate equivalent protein loading in different gel lanes, typically transfected vs mock or non transfected cell lysate.

Gene ID: 60

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction