ALDH1A1 antibody (AA 315-434)

Catalog No. ABIN3024144

The Mouse Monoclonal anti-ALDH1A1 antibody has been validated for WB, IF, FACS and IHC (p). It is suitable to detect ALDH1A1 in samples from Human.

Quick Overview for ALDH1A1 antibody (AA 315-434) (ABIN3024144)

Target: ALDH1A1 (Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ALDH1A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: AHDH1-1

Product Details anti-ALDH1A1 Antibody

Binding Specificity: AA 315-434

Purification: Protein G affinity chromatography

Immunogen: A human partial recombinant ALDH1A1 protein (AA 315-434) was used as the immunogen for this ALDH1 antibody.

Isotype: IgG1

Application Details

Application Notes: Titering of the ALDH1 antibody may be required for optimal performance.

1. FFPE testing requires sections to be boiled in  pH 6 10  mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.\. FACS: 0.5-1 μg/10^6 cells,Western blot: 1-2 μg/mL,IF: 1-2 μg/mL,IHC (FFPE): 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: The ALDH1 antibody (with azide) can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for ALDH1A1

Target: ALDH1A1 (Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1))

Alternative Name: ALDH1

Background: ALDH1A1 belongs to the ALDH enzymes, a family of evolutionarily conserved enzymes comprised of 19 isoforms that are localized in the cytoplasm, mitochondria or nucleus. ALDH1 is predominantly expressed in the epithelium of testis, brain, eye, liver, kidney, as well as neural and hematopoietic stem cells. Reportedly, high ALDH1 expression is found in solitary fibrous tumor (SFT) and hemangiopericytoma (HPC), compared to meningiomas and synovial sarcomas. In combination with CD34, ALDH1 may be useful for the differentiation among SFT, HPC, meningioma, and synovial sarcoma.

Gene ID: 216

Pathways: Dopaminergic Neurogenesis