CD16 antibody

Catalog No. ABIN3025165

This anti-CD16 antibody is a Mouse Monoclonal antibody detecting CD16 in FACS and IF. Suitable for Human.

Quick Overview for CD16 antibody (ABIN3025165)

Target: CD16

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD16 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: ICO-116

Product Details anti-CD16 Antibody

Characteristics: It recognizes CD16 (Fcgamma,RIII), the low-affinity receptor for IgG with an apparent molecular weight of 50-80 kDa. Two similar genes represent CD16, CD16A (Fcgamma,RIIIA), which exists as a hetero-oligomeric polypeptide-anchored form in macrophages and NK cells and CD16B (Fcgamma,RIIIB), which exist as a monomeric GPI-anchored form in neutrophils. Furthermore, there are two known polymorphisms of CD16B, NA-1 and NA-2. Individuals homozygous for NA-2 show a lower phagocytic capacity compared with NA-1. CD16 binds IgG in the form of immune complexes and shows preferential binding of IgG1 and IgG3 isotypes and minimal binding of IgG2 and IgG4. Upon IgG binding, both CD16 isoforms initiate signal transduction cascades that lead to a variety of responses including antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, degranulation and proliferation.

Purification: Protein G affinity chromatography

Immunogen: Human recombinant protein was used as the immunogen for the CD16 antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal dilution of the CD16 antibody should be determined by the researcher.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the CD16 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for CD16

Target: CD16

Alternative Name: CD16

Background: It recognizes CD16 (Fcgamma,RIII), the low-affinity receptor for IgG with an apparent molecular weight of 50-80  kDa. Two similar genes represent CD16, CD16A (Fcgamma,RIIIA), which exists as a hetero-oligomeric polypeptide-anchored form in macrophages and NK cells and CD16B (Fcgamma,RIIIB), which exist as a monomeric GPI-anchored form in neutrophils. Furthermore, there are two known polymorphisms of CD16B, NA-1 and NA-2. Individuals homozygous for NA-2 show a lower phagocytic capacity compared with NA-1. CD16 binds IgG in the form of immune complexes and shows preferential binding of IgG1 and IgG3 isotypes and minimal binding of IgG2 and IgG4. Upon IgG binding, both CD16 isoforms initiate signal transduction cascades that lead to a variety of responses including antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, degranulation and proliferation.

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process