Keratin 10 antibody

Catalog No. ABIN3025176

The Mouse Monoclonal anti-Keratin 10 antibody has been validated for FACS, IF and IHC (p). It is suitable to detect Keratin 10 in samples from Human, Dog and Cat.

Quick Overview for Keratin 10 antibody (ABIN3025176)

Target: Keratin 10 (KRT10)

Reactivity: Human, Dog, Cat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Keratin 10 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: DE-K10

Product Details anti-Keratin 10 Antibody

Purification: Protein G affinity chromatography

Immunogen: A cytoskeletal preparation from human ectocervical epithelium was used as the immunogen for this Cytokeratin 10 antibody.

Isotype: IgG1

Application Details

Application Notes: Optimal dilution of the Cytokeratin 10 antibody should be determined by the researcher.

1. Staining of FFPE tissue requires boiling sections in 10  mM citrate buffer,  pH 6, for 10-20 min followed by cooling at RT for 20 min.\. FACS: 0.5-1 μg/million cells,IF: 0.5-1 μg/mL,IHC (FFPE): 0.1-0.2 μg/mL for 30 minutes at RT (1)

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the Cytokeratin 10 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for Keratin 10

Target: Keratin 10 (KRT10)

Alternative Name: Cytokeratin 10

Background: Keratin-10 is a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in this gene are associated with epidermolytic hyperkeratosis. [Wiki]

Gene ID: 3858