Transferrin antibody

Catalog No. ABIN3025628

This Mouse Monoclonal antibody specifically detects Transferrin in IHC (p) and IF. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for Transferrin antibody (ABIN3025628)

Target: Transferrin (TF)

Reactivity: Human, Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Transferrin antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Clone: SPM320

Product Details anti-Transferrin Antibody

Purification: PEG precipitation

Immunogen: Neuraminidase-treated human red blood cells were used as the immunogen for the CD176 antibody.

Isotype: IgM kappa

Application Details

Application Notes: Optimal dilution of the CD176 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min.\. Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 3-5 μg/mL for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the CD176 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for Transferrin

Target: Transferrin (TF)

Alternative Name: CD176

Background: Recognizes a disaccharide epitope, Gal1-3GalNAc, of Thomsen-Friedenreich (TF) antigen. It is specific for both anomeric forms of the disaccharide (TF and TF, including related structures on the glycolipid) and shows no cross-reactivity with sialylated glycophorin. The Thomsen-Friedenreich antigen acts as an oncofetal antigen, with low expression in normal adult tissues but increasing to fetal levels of expression in hyperplasia or malignancy. It is considered as a pan-carcinoma marker. During metastasis, the ability of malignant cells to form multicellular aggregates via homotypic or heterotypic aggregation and their adhesion to the endothelium are critical. The tumor-associated carbohydrate Thomsen-Friedenreich antigen (Gal-GalNAc) is involved in tumor cell adhesion and tissue invasion. It also causes an immune response, and overexpression of the antigen causes cancer cells to be more sensitive to natural killer cell lysis. The Thomsen-Friedenreich antigen is suppressed in normal healthy cells and represents one of the few chemically well-defined antigens associated with tumor malignancy. The presence of the Thomsen-Friedenreich antigen on the surface of cancer cells may result from a divergence from the normal pathway for O-linked glycosylation in these cells, most likely caused by inappropriate localization of the enzymes involved in synthesis of the disaccharide.

Pathways: Transition Metal Ion Homeostasis