Immunohistochemistry on paraffin sections (10 μg/mL). Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100C for 20 min. Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1xTBS with Tween (TBST) for 1 min. at room temperature. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody at 2 °C to 8 °C up to one month or (in aliquots) at -20 °C to -70 °C for longer.
Target
WNT10A
(Wingless-Type MMTV Integration Site Family, Member 10A (WNT10A))
Alternative Name
WNT10A
Background
Wnts are important signaling molecules involved in normal developmental processes as well as some forms of cancer. WNT10A expression has been documented in many cancers, include those of the stomach, colon, breast, bone marrow, esophagus, and blood.Synonyms: Protein Wnt-10a