SERCA2 ATPase (Middle Region) antibody
Reactivity: Mouse, Rat, Human
WB, IHC (p)
Host: Rabbit
Polyclonal
unconjugated
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- Target
- SERCA2 ATPase
- Binding Specificity
- Middle Region
- Reactivity
- Mouse, Rat, Human
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- Un-conjugated
-
Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Purification
- Antigen affinity
- Immunogen
- An amino acid sequence from the middle region of human SERCA2 ATPase (QRDACLNARCFARVE) was used as the immunogen for this SERCA2 ATPase antibody (100% homologous in human, mouse and rat).
- Isotype
- IgG
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anti-SERCA2 ATPase antibody
Reactivity: Mouse, Rat WB, IF, IHC Host: Rabbit Polyclonal unconjugated
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- Application Notes
- The stated application concentrations are suggested starting amounts. Titration of the SERCA2 ATPase antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL
- Restrictions
- For Research Use only
-
- Buffer
- 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
- Storage
- -20 °C
- Storage Comment
- After reconstitution, the SERCA2 ATPase antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
-
- Target
- SERCA2 ATPase
- Background
- Sarcoplasmic reticulum Ca(2+)-ATPase 2, also called ATP2A2, ATP2B, encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. They are closely related to the plasma membrane Ca(2+)-ATPases, or PMCAs. SERCA2 belongs to the large family of P-type cation pumps that couple ATP hydrolysis with cation transport across membranes. The gene is mapped on 12q24.11. SERCA2 was expressed in all specimens, with pronounced expression in the subnuclear aspect of basal epidermal keratinocytes. There was variable suprabasal expression. Expression was also observed in the infundibulum and outer root sheath of hair follicles, germinative and mature cells of sebaceous glands, secretory coil and duct of eccrine glands, apocrine gland cells, and arrector pili muscle. In Darier disease skin, strong SERCA2 positivity was detected in the basal, suprabasal, and acantholytic lesional cells. Perilesional Darier disease skin was comparable to normal skin.
- UniProt
- P16615
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