Calpain antibody (Catalytic Subunit)

Catalog No. ABIN3030266

The Rabbit Polyclonal anti-Calpain antibody has been validated for WB and IHC (p). It is suitable to detect Calpain in samples from Human, Mouse and Rat.

Quick Overview for Calpain antibody (Catalytic Subunit) (ABIN3030266)

Target: Calpain

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Calpain antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Calpain Antibody

Binding Specificity: AA 396-555, Catalytic Subunit

Purification: Antigen affinity

Immunogen: Human recombinant protein from the CAPN1 catalytic subunit (AA 396-555) was used as the immunogen for this Calpain antibody.

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the Calpain antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the Calpain antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for Calpain

Target: Calpain

Background: Calpain 1 is also known as CAPN, CAPN1 and muCL. The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. The CPN1 gene encodes the catalytic subunit of the ubiquitous enzyme, Calpain 1. Several transcript variants encoding two different isoforms have been found for this gene.

Gene ID: 823