MLF1IP antibody (pThr78)

Catalog No. ABIN303040

This Rabbit Polyclonal antibody specifically detects MLF1IP in WB, IHC (p), EIA and IP. It exhibits reactivity toward Human, Cow, Mouse, Dog, Rat and Monkey.

Quick Overview for MLF1IP antibody (pThr78) (ABIN303040)

Target: MLF1IP (MLF1 Interacting Protein (MLF1IP))

Reactivity: Human, Cow, Mouse, Dog, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MLF1IP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Immunoprecipitation (IP)

Product Details anti-MLF1IP Antibody

Binding Specificity: pThr78

Specificity: This antibody reacts to the amino acids surrounding Thr78 of MLF1IP protein.

Purification: Immunoaffinity Chromatography

Immunogen: Synthetic peptide

Application Details

Application Notes: Immunoprecipitation. Immunohistochemistry: 10 μg/mL. Western Blot: 1: 500 - 1: 2000. ELISA: 1: 5000 - 1: 25000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for MLF1IP

Target: MLF1IP (MLF1 Interacting Protein (MLF1IP))

Alternative Name: MLF1IP

Background: Myeloid leukemia factor-1 (MLF1) Interacting Protein (also known as PBIP1, MLF1IP1, KLIP1 or KSHV latent nuclear antigen interacting protein 1) is a novel polo-like kinase 1 (Plk1) substrate. Plk1 phosphorylation of MLF1IP induces ubiquitination and degradation of MLF1IP prior to the metaphase/anaphase transition. Several Plk1-dependent phosphorylation sites have been identified on MLF1IP by mass spectrometry. Mutations of these sites stabilize MLF1IP and inhibit mitotic progression. Subsequent in vitro and in vivo MLF1IP phosphorylation and stability assays have revealed that phosphorylation of Thr78 is critical for triggering Plk1-dependent MLF1IP degradation. Expression of a non-degradable Thr78Ala mutant was sufficient to induce a mitotic block. Timely phosphorylation of MLF1IP on Thr78 by Plk1 is critical for eliminating the MLF1IP-imposed mitotic block prior to anaphase onset. MLF1IP is speculated to be a novel tumor suppressor, whose function is required for proper sister-chromatid separation. Loss of MLF1IP function may result in improper segregation of chromosomes and genomic instability, thus promoting tumorigenesis.Synonyms: CENP-U(50), CENPU, Centromere protein U, ICEN24, Interphase centromere complex protein 24, KLIP1, KSHV latent nuclear antigen-interacting protein 1, MLF1-interacting protein, PBIP, Polo-box-interacting protein 1

Gene ID: 79682

NCBI Accession: NP_078905

UniProt: Q71F23