HRH3 antibody (C-Term)

Catalog No. ABIN3031207

This Rabbit Polyclonal antibody specifically detects HRH3 in WB. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for HRH3 antibody (C-Term) (ABIN3031207)

Target: HRH3 (Histamine Receptor H3 (HRH3))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HRH3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-HRH3 Antibody

Binding Specificity: C-Term

Purification: Antigen affinity

Immunogen: An amino acid sequence from the C-terminus of human HRH3 (CPQKLKIQPHSSLEHCWK) was used as the immunogen for this HRH3 antibody.

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the HRH3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the HRH3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for HRH3

Target: HRH3 (Histamine Receptor H3 (HRH3))

Alternative Name: HRH3

Background: The histamine receptor H3(HRH3) is a presynaptic autoreceptor on histamine neurons in the brain and a presynaptic heteroreceptor in nonhistamine-containing neurons in both the central and peripheral nervous systems. The deduced 445-amino acid HRH3 protein contains 7 predicted transmembrane domains. And it shares 22 % and 21.4 % amino acid sequence homology with the H1(HRH1) and H2(HRH2) receptors, respectively. The expression of recombinant HRH3 in a variety of cell lines conferred an ability to inhibit adenylate cyclase in response to histamine, but not to acetylcholine or any other biogenic amine. Additionally, HRH3 was most notably observed throughout the thalamus, the ventromedial hypothalamus, and the caudate nucleus. Strong expression was also seen in layers II, V, and VIb of the cerebral cortex, in the pyramidal layers of the hippocampus, and in olfactory tubercle.

UniProt: Q9Y5N1

Pathways: cAMP Metabolic Process, Feeding Behaviour