Lamin B1 antibody (AA 570-586)

Catalog No. ABIN3031586

The Rabbit Polyclonal anti-Lamin B1 antibody has been validated for WB, ICC, IHC (p) and IHC (fro). It is suitable to detect Lamin B1 in samples from Human and Rat.

Quick Overview for Lamin B1 antibody (AA 570-586) (ABIN3031586)

Target: Lamin B1 (LMNB1)

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Lamin B1 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Lamin B1 Antibody

Binding Specificity: AA 570-586

Purification: Antigen affinity

Immunogen: Amino acids 570-586 (FHQQGTPRASNRSCAIM-human) were used as the immunogen for this Lamin B1 antibody.

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the Lamin B1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL,IHC (Frozen): 0.5-1 μg/mL,Immunocytochemistry: 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the Lamin B1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for Lamin B1

Target: Lamin B1 (LMNB1)

Alternative Name: Lamin B1

Background: Lamin B1 is a protein that in humans is encoded by the LMNB1 gene. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. They are though to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1.

UniProt: P20700

Pathways: Apoptosis, Caspase Cascade in Apoptosis