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CARD9 antibody (AA 521-536)

This Rabbit Polyclonal antibody specifically detects CARD9 in WB, IF and IHC (p). It exhibits reactivity toward Human.
Catalog No. ABIN303199

Quick Overview for CARD9 antibody (AA 521-536) (ABIN303199)

Target

See all CARD9 Antibodies
CARD9 (Caspase Recruitment Domain Family, Member 9 (CARD9))

Reactivity

  • 45
  • 5
  • 3
  • 2
  • 2
  • 1
Human

Host

  • 44
  • 3
Rabbit

Clonality

  • 45
  • 2
Polyclonal

Conjugate

  • 27
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This CARD9 antibody is un-conjugated

Application

  • 40
  • 13
  • 13
  • 13
  • 11
  • 10
  • 7
  • 6
  • 3
  • 3
  • 1
  • 1
  • 1
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • Binding Specificity

    • 15
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    AA 521-536

    Sequence

    DRENTTGSDN TDTEGS

    Cross-Reactivity (Details)

    Species reactivity (tested):Human.

    Purification

    Immunoaffinity Chromatography.

    Immunogen

    Synthetic peptide - KLH conjugated corresponding to Amino Acids 521-536 of Human CARD9. The sequence is different from that of Rat origin by two Amino Acids.
  • Application Notes

    Western Blot (0.5-1 μg/mL). Immunocytochemistry. Immunohistochemistry on Paraffin Sections (10 μg/mL). This antibody was validated for use in immunohistochemistry on a panel of 21formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigenretrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides wereincubated with biotinylated secondary antibody LS-D1, followed by alkalinephosphatase-streptavidin and chromogen.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user. Species Reactivity: Tested: Human.

    Restrictions

    For Research Use only
  • Concentration

    1.0 mg/mL

    Buffer

    PBS containing 0.02 % Sodium Azide as preservative.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Avoid repeated freezing and thawing.

    Storage

    4 °C/-20 °C

    Storage Comment

    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C to -70 °C for longer. Dilute only prior to immediate use.
  • Target

    CARD9 (Caspase Recruitment Domain Family, Member 9 (CARD9))

    Alternative Name

    CARD9

    Background

    Apoptosis is related to many diseases and development. Cell death signals are transduced by death domain (DD), death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. CARD containing proteins include some caspases, Apaf-1, CARD4, IAPs, RICK, ARC, RAIDD, BCL-10, and ASC. A novel CARD-containing protein was recently identified and designated CARD9, which interacts with the CARD activation domain of BCL-10. CARD9 associates with BCL-10 and forms a complex within cells. CARD9 induces apoptosis and activates NF-kB. CARD9 is an upstream activator of BCL-10 and NF-kB signaling.Synonyms: Caspase recruitment domain-containing protein 9

    Gene ID

    64170

    NCBI Accession

    NP_434700

    UniProt

    Q9H257

    Pathways

    Activation of Innate immune Response
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