PFN2 antibody (C-Term)

Catalog No. ABIN3032305

This Rabbit Polyclonal antibody specifically detects PFN2 in WB and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for PFN2 antibody (C-Term) (ABIN3032305)

Target: PFN2 (Profilin 2 (PFN2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PFN2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PFN2 Antibody

Binding Specificity: C-Term

Purification: Antigen affinity

Immunogen: An amino acid sequence from the C-terminus of human PFN2 (NKKAYSMAKYLRDSGF) was used as the immunogen for this Profilin 2 antibody (100% homologous in human, mouse and rat).

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the Profilin 2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the Profilin 2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for PFN2

Target: PFN2 (Profilin 2 (PFN2))

Alternative Name: Profilin 2

Background: Profilin-2 is a protein that in humans is encoded by the PFN2 gene. The protein encoded by this gene is a ubiquitous actin monomer-binding protein belonging to the profilin family. This gene is mapped to 3q25.1. It is thought to regulate actin polymerization in response to extracellular signals. There are two alternatively spliced transcript variants encoding different isoforms described for this gene. Profilin-2 binds to actin and affects the structure of the cytoskeleton. At high concentrations, it prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG.

UniProt: P35080

Pathways: Regulation of Actin Filament Polymerization, Synaptic Vesicle Exocytosis