SLC6A1 antibody (C-Term)

Catalog No. ABIN3032687

The Rabbit Polyclonal anti-SLC6A1 antibody has been validated for WB and IHC (p). It is suitable to detect SLC6A1 in samples from Human, Rat and Mouse.

Quick Overview for SLC6A1 antibody (C-Term) (ABIN3032687)

Target: SLC6A1 (GAT1) (GABA Transporter 1 (GAT1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC6A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SLC6A1 Antibody

Binding Specificity: C-Term

Purification: Antigen affinity

Immunogen: An amino acid sequence from the C-terminus of human GABA Transporter 1 (WFYGVNRFYDNIQEM) was used as the immunogen for this GAT-1 antibody (100% homologous in human, mouse and rat).

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the GAT-1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the GAT-1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for SLC6A1

Target: SLC6A1 (GAT1) (GABA Transporter 1 (GAT1))

Alternative Name: GAT-1

Background: SLC6A1 (Solute Carrier family 6(Neurotransmitter transporter, Gaba), Member 1), also called GABATR, GABATHG and GAT-1, encodes a gamma-aminobutyric acid (GABA) transporter, which removes GABA from the synaptic cleft. Zomot et al.(2007) showed that introduction of a negatively charged amino acid at or near one of the two putative sodium binding sites of GAT-1 from rat brain renders both net flux and exchange of GABA largely chloride independent. By in vitro functional expression studies, the GAT-1 insertion promoter (two copies of the sequence) had significantly greater promoter activity than the noninsertion (one copy of the sequence) promoter, which in turn had greater activity than a promoterless control. Studies of postmortem hippocampus from 69 African American individuals showed that overall expression decreased with age.

UniProt: P30531