CD53 antibody (PE)

Catalog No. ABIN303518

The Mouse Monoclonal anti-CD53 antibody has been validated for FACS. It is suitable to detect CD53 in samples from Human.

Quick Overview for CD53 antibody (PE) (ABIN303518)

Target: CD53

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD53 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Clone: MEM-53

Product Details anti-CD53 Antibody (PE)

Purification: Size-exclusion chromatography

Immunogen: Leukocytes of pacient suffering from a LGL-type leukemia

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry analysis of human blood cells using 20 μL reagent / 100 μL of whole blood or 10^6 cells in a suspension.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: Phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2 % (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Dilute only prior to immediate use

Storage: 4 °C

Storage Comment: Store the antibody (undiluted) at 2-8 °C. DO NOT FREEZE! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Target Details for CD53

Target: CD53

Alternative Name: CD53

Background: CD53 is a tetraspanin family transmembrane glycoprotein expressed in the lymphoid-myeloid lineage. This molecule has been reported to form complexes with other leukocyte surface proteins such as CD2, CD19, CD21, MHC II, VLA-4 or tetraspanins CD37, CD81 and CD82, thus probably modulating various signaling processes. CD53 is involved in radioresistancy of tumour cells and its triggering has anti-apoptotic effect. In thymus, CD53 is up-regulated in response to positive selection signals during T cell development, and is strongly expressed upon macrophage exposure to bacterial lipopolysaccharide, whereas stimulation of neutrophils results in down-regulation of CD53 expression.Synonyms: Cell surface glycoprotein CD53, Leukocyte surface antigen CD53, MOX44, TSPAN25, Tetraspanin-25

Gene ID: 963

NCBI Accession: NP_000551

UniProt: P19397