Butyrylcholinesterase antibody (AA 263-571)

Catalog No. ABIN3042329

The Rabbit Polyclonal anti-Butyrylcholinesterase antibody has been validated for WB. It is suitable to detect Butyrylcholinesterase in samples from Mouse.

Quick Overview for Butyrylcholinesterase antibody (AA 263-571) (ABIN3042329)

Target: Butyrylcholinesterase (BCHE)

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Butyrylcholinesterase antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Butyrylcholinesterase Antibody

Binding Specificity: AA 263-571

Purpose: Anti-Butyrylcholinesterase/BCHE Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Butyrylcholinesterase/BCHE Antibody Picoband® (ABIN3042329). Tested in WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived mouse BCHE recombinant protein (Position: K263-E571). Mouse BCHE shares 78% amino acid (aa) sequence identity with human BCHE.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Mouse
1. Allderdice PW, Gardner HA, Galutira D, Lockridge O, LaDu BN, McAlpine PJ (Oct 1991). "The cloned butyrylcholinesterase (BCHE) gene maps to a single chromosome site, 3q26". Genomics 11 (2): 452-4. 2. Jasmin L (2013-05-28). "Cholinesterase - blood". University of Maryland Medical Center. 3. Pohanka M (2013). "Butyrylcholinesterase as a biochemical marker". Bratislavské Lekárske Listy 114 (12): 726-734.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for Butyrylcholinesterase

Target: Butyrylcholinesterase (BCHE)

Alternative Name: Bche

Background:

Synonyms: Cholinesterase,3.1.1.8,Acylcholine acylhydrolase,Butyrylcholine esterase,Choline esterase II,Pseudocholinesterase,Bche,

Tissue Specificity: Present in most cells except erythrocytes.

Background: Butyrylcholinesterase (BCHE, or BuChE), also known as pseudocholinesterase or plasma cholinesterase, is a non-specific cholinesterase enzyme that hydrolyses many different choline esters. In humans, it is found primarily in the liver and is encoded by the BCHE gene. Mutant alleles at the BCHE locus are responsible for suxamethonium sensitivity. Homozygous persons sustain prolonged apnea after administration of the muscle relaxant suxamethonium in connection with surgical anesthesia. The activity of pseudocholinesterase in the serum is low and its substrate behavior is atypical. In the absence of the relaxant, the homozygote is at no known disadvantage.

Sequence Similarities: Contains 1 CARD domain.

Molecular Weight: 85-90 kDa

Gene ID: 12038

UniProt: Q03311

Pathways: Peptide Hormone Metabolism