MGST1 antibody (N-Term)

Catalog No. ABIN3042364

This Rabbit Polyclonal antibody specifically detects MGST1 in WB and IHC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for MGST1 antibody (N-Term) (ABIN3042364)

Target: MGST1 (Microsomal Glutathione S-Transferase 1 (MGST1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MGST1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-MGST1 Antibody

Binding Specificity: AA 42-75, N-Term

Purpose: Anti-Microsomal Glutathione S-transferase 1/MGST1 Antibody Picoband®

Sequence: KVFANPEDCV AFGKGENAKK YLRTDDRVER VRRA

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Microsomal Glutathione S-transferase 1/MGST1 Antibody Picoband® (ABIN3042364). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human MGST1, different from the related mouse sequence by six amino acids, and from the related rat sequence by five amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry, 2-5 μg/mL, Human1. Lee SH, DeJong J (1999). "Microsomal GST-I: genomic organization, expression, and alternative splicing of the human gene.". Biochim. Biophys. Acta 1446 (3): 389-96. 2. "Entrez Gene: MGST1 microsomal glutathione S-transferase 1".

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for MGST1

Target: MGST1 (Microsomal Glutathione S-Transferase 1 (MGST1))

Alternative Name: MGST1

Background:

Synonyms: Microsomal glutathione S-transferase 1,Microsomal GST-1,2.5.1.18,Microsomal GST-I,MGST1,GST12, MGST,

Tissue Specificity: Highly expressed in liver.

Background: Microsomal glutathione S-transferase 1 is an enzyme that in humans is encoded by the MGST1 gene. The MAPEG (Membrane Associated Proteins in Eicosanoid and Glutathione metabolism) family consists of six human proteins, two of which are involved in the production of leukotrienes and prostaglandin E, important mediators of inflammation. Other family members, demonstrating glutathione S-transferase and peroxidase activities, are involved in cellular defense against toxic, carcinogenic, and pharmacologically active electrophilic compounds. This gene encodes a protein that catalyzes the conjugation of glutathione to electrophiles and the reduction of lipid hydroperoxides. This protein is localized to the endoplasmic reticulum and outer mitochondrial membrane where it is thought to protect these membranes from oxidative stress. Several transcript variants, some non-protein coding and some protein coding, have been found for this gene.

Molecular Weight: 15 kDa

Gene ID: 4257

UniProt: P10620