FUT1 antibody (N-Term)

Catalog No. ABIN3042410

The Rabbit Polyclonal anti-FUT1 antibody has been validated for WB and IHC. It is suitable to detect FUT1 in samples from Human, Mouse and Rat.

Quick Overview for FUT1 antibody (N-Term) (ABIN3042410)

Target: FUT1 (Fucosyltransferase 1 (Galactoside 2-alpha-L-Fucosyltransferase, H Blood Group) (FUT1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FUT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-FUT1 Antibody

Binding Specificity: AA 134-164, N-Term

Purpose: Anti-Galactoside 2-alpha-L-fucosyltransferase 1/FUT1 Antibody Picoband®

Sequence: EVDSRTPWRE LQLHDWMSEE YADLRDPFLK L

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Galactoside 2-alpha-L-fucosyltransferase 1/FUT1 Antibody Picoband® (ABIN3042410). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human FUT1, different from the related mouse and rat sequences by seven amino acids.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Western blot, 0.1-0.5 μg/mL, Human, Rat
1. "Entrez Gene: FUT1 fucosyltransferase 1 (galactoside 2-alpha-L-fucosyltransferase, H blood group)". 2. Ball SP, Tongue N, Gibaud A et al. (1992). "The human chromosome 19 linkage group FUT1 (H), FUT2 (SE), LE, LU, PEPD, C3, APOC2, D19S7 and D19S9". Ann. Hum. Genet. 55 (Pt 3): 225-33. 3. Yip SP, Chee KY, Chan PY et al. (2003). "Molecular genetic analysis of para-Bombay phenotypes in Chinese: a novel non-functional FUT1 allele is identified". Vox Sang. 83 (3): 258-62.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for FUT1

Target: FUT1 (Fucosyltransferase 1 (Galactoside 2-alpha-L-Fucosyltransferase, H Blood Group) (FUT1))

Alternative Name: FUT1

Background:

Synonyms: Galactoside 2-alpha-L-fucosyltransferase 1,2.4.1.69,Alpha (1,2)FT 1,Blood group H alpha 2-fucosyltransferase,Fucosyltransferase 1,GDP-L-fucose:beta-D-galactoside 2-alpha-L-fucosyltransferase 1,FUT1,H, HSC,

Tissue Specificity: Ubiquitous.

Background: Galactoside 2-alpha-L-fucosyltransferase 1 is an enzyme that in humans is encoded by the FUT1 gene. It is mapped to 19q13.3. The protein encoded by this gene is a Golgi stack membrane protein that is involved in the creation of a precursor of the H antigen, which is required for the final step in the soluble A and B antigen synthesis pathway. This gene is one of two encoding the galactoside 2-L-fucosyltransferase enzyme. Mutations in this gene are a cause of the H-Bombay blood group.

Sequence Similarities: Belongs to the glycosyltransferase 11 family.

Molecular Weight: 50 kDa

Gene ID: 2523

UniProt: P19526