FZD1 antibody (C-Term)

Catalog No. ABIN3042412

This Rabbit Polyclonal antibody specifically detects FZD1 in WB and IHC. It exhibits reactivity toward Human.

Quick Overview for FZD1 antibody (C-Term) (ABIN3042412)

Target: FZD1 (Fzd1) (Frizzled Family Receptor 1 (Fzd1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FZD1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-FZD1 Antibody

Binding Specificity: AA 369-400, C-Term

Purpose: Anti-Frizzled homolog 1/FZD1 Antibody Picoband®

Sequence: YIAGFLLEDR VVCNDKFAED GARTVAQG

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Frizzled homolog 1/FZD1 Antibody Picoband® (ABIN3042412). Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Frizzled homolog 1, identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-2 μg/mL, Human
Western blot, 0.1-0.6 μg/mL, Human
1. "Entrez Gene: FZD1 frizzled homolog 1 (Drosophila)". 2. Gazit A, Yaniv A, Bafico A, Pramila T, Igarashi M, Kitajewski J, Aaronson SA (Oct 1999). "Human frizzled 1 interacts with transforming Wnts to transduce a TCF dependent transcriptional response". Oncogene 18(44): 5959-66. 3. Sagara N, Toda G, Hirai M, Terada M, Katoh M (Nov 1998). "Molecular cloning, differential expression, and chromosomal localization of human frizzled-1, frizzled-2, and frizzled-7". Biochemical and Biophysical Research Communications 252 (1): 117-22.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for FZD1

Target: FZD1 (Fzd1) (Frizzled Family Receptor 1 (Fzd1))

Alternative Name: FZD1

Background:

Synonyms: Frizzled-1,Fz-1,hFz1,FzE1,FZD1,

Tissue Specificity: Expressed in adult heart, placenta, lung, kidney, pancreas, prostate, and ovary and in fetal lung and kidney.

Background: Frizzled-1 (Frizzled homolog 1) is a protein that in humans is encoded by the FZD1 gene. Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signalingproteins. The FZD1 protein contains a signal peptide, a cysteine-rich domain in the N-terminal extracellular region, 7 transmembrane domains, and a C-terminal PDZ domain-binding motif. The FZD1 transcript is expressed in various tissues.

Sequence Similarities: Belongs to the G-protein coupled receptor Fz/Smo family.

Molecular Weight: 71 kDa

Gene ID: 8321

Pathways: WNT Signaling, Asymmetric Protein Localization