HDAC6 antibody (N-Term)

Catalog No. ABIN3042440

The Rabbit Polyclonal anti-HDAC6 antibody has been validated for WB and FACS. It is suitable to detect HDAC6 in samples from Human, Mouse and Rat.

Quick Overview for HDAC6 antibody (N-Term) (ABIN3042440)

Target: HDAC6 (Histone Deacetylase 6 (HDAC6))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HDAC6 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-HDAC6 Antibody

Binding Specificity: AA 137-169, N-Term

Purpose: Anti-HDAC6 Antibody Picoband®

Sequence: EKEELMLVHS LEYIDLMETT QYMNEGELRV LAD

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-HDAC6 Antibody Picoband® (ABIN3042440). Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human HDAC6, different from the related mouse sequence by one amino acid.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human
1. Bertos, N. R., Gilquin, B., Chan, G. K. T., Yen, T. J., Khochbin, S., Yang, X.-J. Role of the tetradecapeptide repeat domain of human histone deacetylase 6 in cytoplasmic retention. J. Biol. Chem. 279: 48246-48254, 2004. 2. Grozinger, C. M., Hassig, C. A., Schreiber, S. L. Three proteins define a class of human histone deacetylases related to yeast Hda1p. Proc. Nat. Acad. Sci. 96: 4868-4873, 1999.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for HDAC6

Target: HDAC6 (Histone Deacetylase 6 (HDAC6))

Alternative Name: HDAC6

Background:

Synonyms: Histone deacetylase 6,HD6,3.5.1.98,HDAC6,KIAA0901,JM21,

Tissue Specificity: Liver.

Background: HDAC6, also called KIAA0901, is a member belongs to class II of the histone deacetylase/acuc/apha family of proteins that is an enzyme that in humans is encoded by the HDAC6 gene. The HDAC6 gene is mapped to chromosome Xp11.23. HDAC6 contains an internal duplication of two catalytic domains which appear to function independently of each other. The protein possesses histone deacetylase activity and represses transcription. HDAC6 functions as a tubulin deacetylase. And it is localized exclusively in the cytoplasm, where it associates with microtubules and localizes with the microtubule motor complex. HDAC6 could bind both polyubiquitinated misfolded proteins and dynein motors, thereby recruiting misfolded protein cargo to dynein motors for transport to aggresomes. Furthermore, expression of HDAC6 was sufficient to rescue degeneration associated with UPS dysfunction in vivo in an autophagy-dependent manner. HDAC6 is a central component of the stress response that regulates SG formation and potentially contributes to control of RNA metabolism and translation.

Sequence Similarities: Contains 1 HMG box DNA-binding domain.

Molecular Weight: 160 kDa

Gene ID: 10013

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling