CCL18 antibody (AA 21-89)

Catalog No. ABIN3042511

This Rabbit Polyclonal antibody specifically detects CCL18 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for CCL18 antibody (AA 21-89) (ABIN3042511)

Target: CCL18 (Chemokine (C-C Motif) Ligand 18 (Pulmonary and Activation-Regulated) (CCL18))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCL18 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-CCL18 Antibody

Binding Specificity: AA 21-89

Purpose: Anti-Macrophage Inflammatory Protein 4/CCL18 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Macrophage Inflammatory Protein 4/CCL18 Antibody Picoband® (ABIN3042511). Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CCL18 recombinant protein (Position: A21-A89).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, -
ELISA(Cap) , 1-5 μg/mL, Human
1. Hieshima k, I. T. (August 1997). "A novel human CC chemokine PARC that is most homologous to macrophage-inflammatory protein-1 alpha/LD78 alpha and chemotactic for T lymphocytes, but not for monocytes". Journal of Immunology 159 (3): 1140-1149. 2. Tasaki, Y., Fukuda, S., Iio, M., Miura, R., Imai, T., Sugano, S., Yoshie, O., Hughes, A. et al. (1999). "Chemokine PARC Gene (SCYA18) Generated by Fusion of Two MIP-1α/LD78α-like Genes, ,". Genomics 55 (3): 353-357. 3. Hieshima, K., Imai, T., Baba, M., Shoudai, K., Ishizuka, K., Nakagawa, T., Tsuruta, J., Takeya, M., Sakaki, Y., Takatsuki, K., Miura, R., Opdenakker, G., Van Damme, J., Yoshie, O., Nomiyama, H. A novel human CC chemokine PARC that is most homologous to macrophage-inflammatory protein-1-alpha/LD78-alpha and chemotactic for T lymphocytes, but not for monocytes. J. Immun. 159: 1140-1149, 1997.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for CCL18

Target: CCL18 (Chemokine (C-C Motif) Ligand 18 (Pulmonary and Activation-Regulated) (CCL18))

Alternative Name: CCL18

Background:

Synonyms: C-C motif chemokine 18,Alternative macrophage activation-associated CC chemokine 1,AMAC-1,CC chemokine PARC,Dendritic cell chemokine 1,DC-CK1,Macrophage inflammatory protein 4,MIP-4,Pulmonary and activation-regulated chemokine,Small-inducible cytokine A18,CCL18 (1-68),CCL18 (3-69),CCL18 (4-69),CCL18,AMAC1, DCCK1, MIP4, PARC, SCYA18,

Tissue Specificity: Expressed at high levels in lung, lymph nodes, placenta, bone marrow, dendritic cells present in germinal centers and T-cell areas of secondary lymphoid organs and macrophages derived from peripheral blood monocytes. Not expressed by peripheral blood monocytes and a monocyte-to-macrophage differentiation is a prerequisite for expression. Expressed in synovial fluids from patients with rheumatoid and septic arthritis and in ovarian carcinoma ascitic fluid. .

Background: Macrophage Inflammatory Protein 4, also known as CCL18, is a small cytokine belonging to the CC chemokine family that was previously called PARC (pulmonary and activation-regulated chemokine). CCL18 is approximately 60 % identical in amino acid sequence to CCL3. By analysis of a previously mapped CCL18 from 17q11.2, it is determined that the PARC gene is located within 1 of the 2 clusters of CC chemokine genes in this region. It is expressed at high levels in lung and at lower levels in certain lymphoid tissues, such as the lymph nodes, and is chemotactic for activated T cells and nonactivated lymphocytes. Beside, CCL18 recruits Th2 cells and basophils and may play a predominant role in allergic asthma.

Sequence Similarities: Belongs to the intercrine beta (chemokine CC) family.

Molecular Weight: 19 kDa

Gene ID: 6362

UniProt: P55774