ADIPOQ antibody (AA 19-244)

Catalog No. ABIN3042521

Product Details anti-ADIPOQ Antibody

Target: ADIPOQ (Adiponectin (ADIPOQ))

Binding Specificity: AA 19-244

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADIPOQ antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Purpose: Anti-Adiponectin/ADIPOQ Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Adiponectin/ADIPOQ Antibody Picoband® (ABIN3042521). Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived rat Adiponectin recombinant protein (Position: I19-N244). Rat Adiponectin shares 90% amino acid (aa) sequence identity with mouse Adiponectin.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Mouse, Rat
Western blot, 0.1-0.5 μg/mL, Rat
1. Das, K., Lin, Y., Widen, E., Zhang, Y., Scherer, P. E. Chromosomal localization, expression pattern, and promoter analysis of the mouse gene encoding adipocyte-specific secretory protein Acrp30. Biochem. Biophys. Res. Commun. 280: 1120-1129, 2001. 2. Schaffler, A., Orso, E., Palitzsch, K.-D., Buchler, C., Drobnik, W., Furst, A., Scholmerich, J., Schmitz, G. The human apM-1, an adipocyte-specific gene linked to the family of TNF's and to genes expressed in activated T cells, is mapped to chromosome 1q21.3-q23, a susceptibility locus identified for familial combined hyperlipidaemia (FCH). Biochem. Biophys. Res. Commun. 260: 416-425, 1999.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-ADIPOQ antibody (ABIN3042521)

Zhang, Xie, Yu, Feng, Wang, Li, Liu: "Constitutive Expression of Adiponectin in Endothelial Progenitor Cells Protects a Rat Model of Cerebral Ischemia." in: Neural plasticity, Vol. 2017, pp. 6809745, (2018) (PubMed).

Qin, Ma, Yang, Hu, Zhou, Fu, Tian, Liu, Xu, Shen: "A Triterpenoid Inhibited Hormone-Induced Adipocyte Differentiation and Alleviated Dexamethasone-Induced Insulin Resistance in 3T3-L1 adipocytes." in: Natural products and bioprospecting, Vol. 5, Issue 3, pp. 159-66, (2015) (PubMed).

Gao, Li, Zhang, Dai, Jiang, Liu, Zhang, Chen, Zhang: "Silencing of ADIPOQ efficiently suppresses preadipocyte differentiation in porcine." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 31, Issue 2-3, pp. 452-61, (2013) (PubMed).

Liu, Mu, Yuan, Wu, Liu, Zheng, Lian, Ren, Xu: "High salt intake fails to enhance plasma adiponectin in normotensive salt-sensitive subjects." in: Nutrition (Burbank, Los Angeles County, Calif.), Vol. 28, Issue 4, pp. 422-5, (2012) (PubMed).

Target Details for ADIPOQ

Target: ADIPOQ (Adiponectin (ADIPOQ))

Alternative Name: ADIPOQ (ADIPOQ Products)

Background:

Synonyms: 30 kDa adipocyte complement-related protein ,Adiponectin, C1Q and collagen domain containing ,Protein Adipoq ,Adipoq ,Acdc ,rCG_36676 ,

Background: Adiponectin, also known as APM1 or ACDC, is a protein which in humans is encoded by the ADIPOQ gene. It is mapped to chromosome 16 in a region showing homology of synteny with human 3q27. By RNase protection and Western blot analysis, it has been showed that Adiponectin is expressed by differentiated adipocytes as a 33-kD protein that is also detectable in serum. By sequence comparisons, it has been found that links between APM1 and TNF family ligands as well as to cytokines expressed by T cells. Adiponectin is a protein hormone that modulates a number of metabolic processes, including glucose regulation and fatty acid oxidation. This gene is exclusively secreted from adipose tissue (and also from the placenta in pregnancy) into the bloodstream and is very abundant in plasma relative to many hormones.

Molecular Weight: 26 kDa

Gene ID: 246253

UniProt: Q8K3R4