CADM1 antibody (N-Term)

Catalog No. ABIN3042543

This anti-CADM1 antibody is a Rabbit Polyclonal antibody detecting CADM1 in WB. Suitable for Human, Mouse and Rat.

Quick Overview for CADM1 antibody (N-Term) (ABIN3042543)

Target: CADM1 (Cell Adhesion Molecule 1 (CADM1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CADM1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CADM1 Antibody

Binding Specificity: AA 73-92, N-Term

Purpose: Anti-SynCAM/CADM1 Antibody Picoband®

Sequence: VIQLLNPNRQ TIYFRDFRPL

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-SynCAM/CADM1 Antibody (ABIN3042543). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human SynCAM, identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Rat, Mouse
1. Biederer, T., Sara, Y., Mozhayeva, M., Atasoy, D., Liu, X., Kavalali, E. T., Sudhof, T. C.SynCAM, a synaptic adhesion molecule that drives synapse assembly. Science 297: 1525-1531, 2002. 2. Gomyo H, Arai Y, Tanigami A, Murakami Y, Hattori M, Hosoda F, Arai K, Aikawa Y, Tsuda H, Hirohashi S, Asakawa S, Shimizu N, Soeda E, Sakaki Y, Ohki M (Feb 2000). "A 2-Mb sequence-ready contig map and a novel immunoglobulin superfamily gene IGSF4 in the LOH region of chromosome 11q23.2". Genomics 62 (2): 139-46. 3. Yageta, Mika, Kuramochi Masami, Masuda Mari, Fukami Takeshi, Fukuhara Hiroshi, Maruyama Tomoko, Shibuya Masabumi, Murakami Yoshinori (Sep 2002). " association of TSLC1 and DAL-1, two distinct tumor suppressor proteins in lung cancer". Cancer Res. (United States) 62 (18): 5129-33.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for CADM1

Target: CADM1 (Cell Adhesion Molecule 1 (CADM1))

Alternative Name: CADM1

Background:

Synonyms: Cell adhesion molecule 1,Immunoglobulin superfamily member 4,IgSF4,Nectin-like protein 2,NECL-2,Spermatogenic immunoglobulin superfamily,SgIgSF,Synaptic cell adhesion molecule,SynCAM,Tumor suppressor in lung cancer 1,TSLC-1,CADM1 ,IGSF4 , IGSF4A, NECL2 , SYNCAM , TSLC1 ,

Tissue Specificity: Expressed in pancreatic islets and in brain, where it has been seen in cerebral cortex, hippocampus, basal ganglia, habenula and thalamus. Expressed in the small cell lung carcinoma cell line SCC-9. No expression in skeletal muscle. .

Background: Cell adhesion molecule 1 is a protein that, in humans, is encoded by the CADM1 gene. This gene is also known as IGSF4, SYNCAM or TSLC1, and it is mapped to 11q23.3. CADM1 functions as a homophilic cell adhesion molecule at the synapse. Expression of the isolated cytoplasmic tail of CADM1 in neurons inhibited synapse assembly. Conversely, expression of full-length CADM1 in nonneuronal cells induced synapse formation by cocultured hippocampal neurons with normal release properties. CADM1 has been shown to interact with EPB41L3.

Sequence Similarities: Belongs to the nectin family.

Molecular Weight: 60 kDa

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Activated T Cell Proliferation