CDC37 antibody (AA 216-378)

Catalog No. ABIN3042602

The Rabbit Polyclonal anti-CDC37 antibody has been validated for WB. It is suitable to detect CDC37 in samples from Human. There is 1 publication available.

Quick Overview for CDC37 antibody (AA 216-378) (ABIN3042602)

Target: CDC37 (Cell Division Cycle 37 Homolog (S. Cerevisiae) (CDC37))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDC37 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CDC37 Antibody

Binding Specificity: AA 216-378

Purpose: Anti-Cdc37 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Cdc37 Antibody Picoband® (ABIN3042602). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Cdc37 recombinant protein (Position: M216-V378). Human Cdc37 shares 95.7% and 95.1% amino acid (aa) sequence identity with mouse and rat Cdc37, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
1. Chen, G., Cao, P., Goeddel, D. V. TNF-induced recruitment and activation of the IKK complex require Cdc37 and Hsp90. Molec. Cell 9: 401-410, 2002. 2. Vaughan, C. K., Mollapour, M., Smith, J. R., Truman, A., Hu, B., Good, V. M., Panaretou, B., Neckers, L., Clarke, P. A., Workman, P., Piper, P. W., Prodromou, C., Pearl, L. H.Hsp90-dependent activation of protein kinases is regulated by chaperone-targeted dephosphorylation of Cdc37. Molec. Cell 31: 886-895, 2008.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-CDC37 antibody (ABIN3042602)

Cheng, Zhao, Wang, Lu, Wang, Wang, Yao: "The effect of 5'-adenylic acid on hepatic proteome of mice radiated by 60Co γ-ray." in: International journal of molecular sciences, Vol. 15, Issue 1, pp. 186-202, (2014) (PubMed).

Target Details for CDC37

Target: CDC37 (Cell Division Cycle 37 Homolog (S. Cerevisiae) (CDC37))

Alternative Name: CDC37

Background:

Synonyms: Hsp90 co-chaperone Cdc37,Hsp90 chaperone protein kinase-targeting subunit,p50Cdc37,Hsp90 co-chaperone Cdc37, N-terminally processed,CDC37,CDC37A,

Tissue Specificity: T-cell and macrophage specific.

Background: Hsp90 co-chaperone Cdc37, also called P50CDC37, is a protein that in humans is encoded by the CDC37 gene. The protein encoded by this gene is highly similar to Cdc 37, a cell division cycle control protein of Sacchromyces cerevisiae. This gene is mapped to 19p13.2. And this protein is a molecular chaperone with specific function in cell signal transduction. It has been shown to form complex with Hsp90 and a variety of protein kinases including CDK4, CDK6, SRC, RAF-1, MOK, as well as eIF2 alpha kinases. It is thought to play a critical role in ing Hsp90 to its target kinases.

Sequence Similarities: Belongs to the CDC37 family.

Molecular Weight: 44 kDa

Gene ID: 11140

UniProt: Q16543