CCL4 antibody (C-Term)

Catalog No. ABIN3042607

This Rabbit Polyclonal antibody specifically detects CCL4 in WB. It exhibits reactivity toward Mouse.

Quick Overview for CCL4 antibody (C-Term) (ABIN3042607)

Target: CCL4 (Chemokine (C-C Motif) Ligand 4 (CCL4))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCL4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CCL4 Antibody

Binding Specificity: AA 74-88, C-Term

Purpose: Anti-Macrophage Inflammatory Protein 1 beta/CCL4 Antibody Picoband®

Sequence: CANPSEPWVT EYMSD

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Macrophage Inflammatory Protein 1 beta/CCL4 Antibody (ABIN3042607). Tested in WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of mouse CCL4.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Mouse
1. Irving SG, Zipfel PF, Balke J, McBride OW, Morton CC, Burd PR, Siebenlist U, Kelly K (June 1990). Nucleic Acids Res. 18 (11): 3261-70. 2. Bystry RS, Aluvihare V, Welch KA, Kallikourdis M, Betz AG (December 2001). "B cells and professional APCs recruit regulatory T cells via CCL4". Nat. Immunol. 2 (12): 1126-32. 3. Cocchi F, DeVico AL, Garzino-Demo A, Arya SK, Gallo RC, Lusso P (December 1995). Science (journal) 270 (5243): 1811-5. 4. Kamin-Lewis R, Abdelwahab SF, Trang C, Baker A, DeVico AL, Gallo RC, Lewis GK (July 2001).

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for CCL4

Target: CCL4 (Chemokine (C-C Motif) Ligand 4 (CCL4))

Alternative Name: CCL4

Background:

Synonyms: C-C motif chemokine 4,Immune activation protein 2,ACT-2,ACT2,Macrophage inflammatory protein 1-beta,MIP-1-beta,Protein H400,SIS-gamma,Small-inducible cytokine A4,Ccl4,Mip1b, Scya4,

Tissue Specificity: Ubiquitous. Isoform Mdm2-A, isoform Mdm2-B, isoform Mdm2-C, isoform Mdm2-D, isoform Mdm2-E, isoform Mdm2-F and isoform Mdm2-G are observed in a range of cancers but absent in normal tissues.

Background: Chemokine (C-C motif) ligand 4, also known as CCL4, is a protein which in humans is encoded by the CCL4 gene. It is a CC chemokine with specificity for CCR5 receptors. It is a chemoattractant for natural killer cells, monocytes and a variety of other immune cells. CCL4 is a major HIV-suppressive factor produced by CD8+ T cells. Performing-low memory CD8+ T cells that normally synthesize MIP-1-beta. Modi et al. (1991) assigned the SCYA4 gene to a slightly more distal location than had Irving et al. (1990): 17q21-q23 rather than 17q11-q21.

Sequence Similarities: Belongs to the MDM2/MDM4 family.

Molecular Weight: 90 kDa

UniProt: P14097