Ectodysplasin A antibody (Middle Region)

Catalog No. ABIN3042668

Product Details anti-Ectodysplasin A Antibody

Target: Ectodysplasin A (EDA)

Binding Specificity: AA 254-269, Middle Region

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ectodysplasin A antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Anti-Ectodysplasin-A EDA Antibody Picoband®

Sequence: HLQGQGSAIQ VKNDLS

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Ectodysplasin-A EDA Antibody (ABIN3042668). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human EDA, identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. Bayes, M., Hartung, A. J., Ezer, S., Pispa, J., Thesleff, I., Srivastava, A. K., Kere, J. The anhidrotic ectodermal dysplasia gene (EDA) undergoes alternative splicing and encodes ectodysplasin-A with deletion mutations in collagenous repeats. Hum. Molec. Genet. 7: 1661-1669, 1998. 2. Durmowicz, M. C., Cui, C.-Y., Schlessinger, D. The EDA gene is a target of, but does not regulate Wnt signaling. Gene 285: 203-211, 2002.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-Ectodysplasin A antibody (ABIN3042668)

Ning, Chen, Li, Wu, Wu, Li, Feng: "X-irradiation for inhibiting glial scar formation in injured spinal cord." in: Neural regeneration research, Vol. 8, Issue 17, pp. 1582-9, (2014) (PubMed).

Target Details for Ectodysplasin A

Target: Ectodysplasin A (EDA)

Alternative Name: EDA (EDA Products)

Background:

Synonyms: Ectodysplasin-A,Ectodermal dysplasia protein,EDA protein,Ectodysplasin-A, membrane form,Ectodysplasin-A, secreted form,EDA,ED1, EDA2,

Tissue Specificity: Not abundant, expressed in specific cell types of ectodermal (but not mesodermal) origin of keratinocytes, hair follicles, sweat glands. Also in adult heart, liver, muscle, pancreas, prostate, fetal liver, uterus, small intestine and umbilical chord. .

Background: Anhidrotic ectodermal dysplasia (EDA) is an X-linked recessive disorder which affects ectodermal structures. Ectodysplasin-A, the protein encoded by the EDA gene, is a member of the tumor necrosis factor ligand superfamily that forms a collagen triple helix, suggesting functions in signal transduction and cell adhesion. Wnt signaling does control EDA gene expression, but ectodysplasin-A does not feedback on the Wnt pathway.

Sequence Similarities: Belongs to the tumor necrosis factor family.

Molecular Weight: 60 kDa

UniProt: Q92838

Pathways: Tube Formation