CDKN1B antibody (AA 10-198)

Catalog No. ABIN3042744

Product Details anti-CDKN1B Antibody

Target: CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))

Binding Specificity: AA 10-198

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDKN1B antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Cyclin-dependent kinase inhibitor 1B(CDKN1B) detection. Tested with WB in Humnan,Rat.

Cross-Reactivity (Details): Predicted Cross Reactivity: human
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Cyclin-dependent kinase inhibitor 1B(CDKN1B) detection. Tested with WB in Humnan,Rat.
Gene Name: cyclin-dependent kinase inhibitor 1B (p27, Kip1)
Protein Name: Cyclin-dependent kinase inhibitor 1B

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human P27 KIP 1 recombinant protein (Position: S10-T198). Human P27 KIP 1 shares 87% amino acid (aa) sequence identity with mouse P27 KIP 1.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Rat, Predicted Species: Human, The detection limit for P27 KIP 1 is approximately 0.25 ng/lane under reducing conditions.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-CDKN1B antibody (ABIN3042744)

Li, Xu, Li, Zou, Saimaiti, Simayi, Wang, Zhang, Yuan, Zhang: "Stat6 activity-related Th2 cytokine profile and tumor growth advantage of human colorectal cancer cells in vitro and in vivo." in: Cellular signalling, Vol. 24, Issue 3, pp. 718-25, (2012) (PubMed).

Wang, Zhao, Wu, Kong, Zhang, Wu, Li, Zhang, Yu, Jin: "Hepatitis B virus induces G1 phase arrest by regulating cell cycle genes in HepG2.2.15 cells." in: Virology journal, Vol. 8, pp. 231, (2011) (PubMed).

Target Details for CDKN1B

Target: CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))

Alternative Name: CDKN1B (CDKN1B Products)

Synonyms: CDKN4 antibody, KIP1 antibody, MEN1B antibody, MEN4 antibody, P27KIP1 antibody, cdkn1b antibody, sb:cb611 antibody, wu:fb63g10 antibody, wu:fb64g10 antibody, wu:fe18e03 antibody, CDKN1B antibody, Kip1 antibody, p27 antibody, FAM14D antibody, ISG12 antibody, ISG12A antibody, P27 antibody, Cdki1b antibody, AA408329 antibody, AI843786 antibody, p27Kip1 antibody, cdkn1bl antibody, cyclin dependent kinase inhibitor 1B antibody, cyclin-dependent kinase inhibitor 1Bb antibody, interferon alpha inducible protein 27 antibody, cyclin-dependent kinase inhibitor 1B antibody, cyclin-dependent kinase inhibitor 1Ba antibody, CDKN1B antibody, cdkn1bb antibody, IFI27 antibody, Cdkn1b antibody, cdkn1ba antibody

Background: Cyclin-dependent kinase inhibitor 1B (p27KIP1), also known as KIP1 or P27, is an enzyme inhibitor that in humans is encoded by the CDKN1B gene. It encodes a protein which belongs to the Cip/Kipfamily of cyclin dependent kinase (Cdk) inhibitor proteins. It is mapped to 12p13.1. p27KIP1 can inhibit both CDK activation and the kinase activity of assembled and activated cyclin-CDK. The function of p27KIP1 is associated with an aggressive phenotype in human breast cancer. Downregulation of p27KIP1 by CK2-alpha-prime is necessary for development of agonist- and stress-induced cardiac hypertrophy.

Synonyms: AA408329 antibody|AI843786 antibody|Cdki1b antibody|CDKN 1B antibody|CDKN 4 antibody|CDKN1B antibody|CDKN4 antibody|CDN1B_HUMAN antibody|Cyclin Dependent Kinase Inhibitor 1B antibody|Cyclin dependent kinase inhibitor p27 antibody|Cyclin-dependent kinase inhibitor 1B (p27, Kip1) antibody|Cyclin-dependent kinase inhibitor 1B antibody|Cyclin-dependent kinase inhibitor p27 antibody|Cyclin-dependent kinase inhibitor p27 Kip1 antibody|KIP 1 antibody|KIP1 antibody|MEN1B antibody|MEN4 antibody|OTTHUMP00000195098 antibody|OTTHUMP00000195099 antibody|p27 antibody|p27 Kip1 antibody|P27-like cyclin-dependent kinase inhibitor antibody|P27KIP1 antibody

Gene ID: 1027

UniProt: P46527

Pathways: Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Sensory Perception of Sound, Mitotic G1-G1/S Phases, DNA Replication, Positive Regulation of Endopeptidase Activity, Synthesis of DNA, Autophagy