Clusterin antibody (AA 228-449)

Catalog No. ABIN3042750

This anti-Clusterin antibody is a Rabbit Polyclonal antibody detecting Clusterin in WB, IHC and ELISA. Suitable for Human.

Quick Overview for Clusterin antibody (AA 228-449) (ABIN3042750)

Target: Clusterin (CLU)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Clusterin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-Clusterin Antibody

Binding Specificity: AA 228-449

Purpose: Anti-Apolipoprotein J/CLU Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Apolipoprotein J/CLU Antibody Picoband® (ABIN3042750). Tested in ELISA, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human Apolipoprotein J recombinant protein (Position: S228-E449). Human Apolipoprotein J shares 76.1 % and 75.2 % amino acid (aa) sequence identity with mouse and rat Apolipoprotein J, respectively.

Isotype: IgG

Application Details

Application Notes: ELISA, 0.1-0.5 μg/mL, -
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Western blot, 0.1-0.5 μg/mL, Human
1. "Entrez Gene: clusterin". 2. Birkenmeier, E. H., Letts, V. A., Frankel, W. N., Magenheimer, B. S., Calvet, J. P. Sulfated glycoprotein-2 (Sgp-2) maps to mouse chromosome 14. Mammalian Genome 4: 131-132, 1993. 3. Han, B. H., DeMattos, R. B., Dugan, L. L., Kim-Han, J. S., Brendza, R. P., Fryer, J. D., Kierson, M., Cirrito, J., Quick, K., Harmony, J. A. K., Aronow, B. J., Holtzman, D. M. Clusterin contributes to caspase-3-independent brain injury following neonatal hypoxia-ischemia. Nature Med. 7: 338-343, 2001. 4. Jones SE, Jomary C (May 2002). "Clusterin". Int. J. Biochem. Cell Biol. 34 (5): 427-431.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for Clusterin

Target: Clusterin (CLU)

Alternative Name: CLU

Background:

Synonyms: Clusterin,Aging-associated gene 4 protein,Apolipoprotein J,Apo-J,Complement cytolysis inhibitor,CLI,Complement-associated protein SP-40,40,Ku70-binding protein 1,NA1/NA2,Testosterone-repressed prostate message 2,TRPM-2,Clusterin beta chain,ApoJalpha,Complement cytolysis inhibitor a chain,Clusterin alpha chain,ApoJbeta,Complement cytolysis inhibitor b chain,CLU,APOJ, CLI, KUB1,AAG4,

Tissue Specificity: Detected in blood plasma, cerebrospinal fluid, milk, seminal plasma and colon mucosa. Detected in the germinal center of colon lymphoid nodules and in colon parasympathetic ganglia of the Auerbach plexus (at protein level). Ubiquitous. Detected in brain, testis, ovary, liver and pancreas, and at lower levels in kidney, heart, spleen and lung. .

Background: Clusterin (apolipoprotein J) is a 75 - 80 kDa disulfide-linked heterodimeric protein associated with the clearance of cellular debris and apoptosis. In humans, clusterin is encoded by the CLU gene. This protein has several synonyms: dimeric acidic glycoprotein (DAG protein), testosterone repressed prostate message-2 (TRPM-2), sulfated glycoprotein-2 (SGP-2) and complement lysis inhibitor (CLI). Clusterin was mapped to mouse chromosome 14. Clusterin is a ubiquitously expressed molecule thought to influence a variety of processes including cell death. In the brain, it accumulates in dying neurons following seizures and hypoxic-ischemic (H-I) injury. clusterin may be a new therapeutic target to modulate non-caspase-dependent neuronal death following acute brain injury.

Sequence Similarities: Belongs to the clusterin family.

Molecular Weight: 82 kDa

Gene ID: 1191

UniProt: P10909

Pathways: Apoptosis, Negative Regulation of intrinsic apoptotic Signaling