G-CSF antibody (AA 38-207)

Catalog No. ABIN3042762

This anti-G-CSF antibody is a Rabbit Polyclonal antibody detecting G-CSF in WB, ELISA and IHC. Suitable for Human. This Primary Antibody has been cited in 1 publication.

Quick Overview for G-CSF antibody (AA 38-207) (ABIN3042762)

Target: G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This G-CSF antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-G-CSF Antibody

Binding Specificity: AA 38-207

Purpose: Anti-G-CSF/CSF3 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-G-CSF/CSF3 Antibody Picoband® (ABIN3042762). Tested in ELISA, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human G-CSF recombinant protein (Position: S38-P207). Human G-CSF shares 76% amino acid (aa) sequence identity with mouse G-CSF.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
Immunohistochemistry, 2-5 μg/mL, Human
ELISA, 0.1-0.5 μg/mL, -
1. Metcalf D (July 1985). "The granulocyte-macrophage colony-stimulating factors". Science 229 (4708): 16-22. 2. Nagata S, Tsuchiya M, Asano S, Kaziro Y, Yamazaki T, Yamamoto O, Hirata Y, Kubota N, Oheda M, Nomura H (1986). "Molecular cloning and expression of cDNA for human granulocyte colony-stimulating factor". Nature 319 (6052): 415-8. 3. Souza LM, Boone TC, Gabrilove J, Lai PH, Zsebo KM, Murdock DC, Chazin VR, Bruszewski J, Lu H, Chen KK, Barendt J, Platzer, E, Moore, MAS, Mertelsmann R, Welte K (April 1986). "Recombinant human granulocyte colony-stimulating factor: effects on normal and leukemic myeloid cells". Science 232 (4746): 61-5.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-G-CSF antibody (ABIN3042762)

Zhang, Wang, Wang, Jiang, Yu, Chong, Fu: "Ferulic acid ameliorates nerve injury induced by cerebral ischemia in rats." in: Experimental and therapeutic medicine, Vol. 9, Issue 3, pp. 972-976, (2015) (PubMed).

Target Details for G-CSF

Target: G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))

Alternative Name: CSF3

Background:

Synonyms: Granulocyte colony-stimulating factor,G-CSF,Pluripoietin,Filgrastim,Lenograstim,CSF3,C17orf33, GCSF,

Tissue Specificity: Non-neural epithelial tissues.

Background: Granulocyte-colony stimulating factor (G-CSF or GCSF), also known as colony-stimulating factor 3 (CSF 3), is a glycoprotein that stimulates the bone marrow to produce granulocytes and stem cells and release them into the bloodstream. Functionally, it is a cytokine and hormone, a type of colony-stimulating factor, and is produced by a number of different tissues. The pharmaceutical analogs of naturally occurring G-CSF are called filgrastim and lenograstim. G-CSF also stimulates the survival, proliferation, differentiation, and function of neutrophil precursors and mature neutrophils.

Sequence Similarities: Belongs to the IL-6 superfamily.

Molecular Weight: 22 kDa

Gene ID: 1440

UniProt: P09919

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization