G-CSF antibody (AA 47-208)

Catalog No. ABIN3042765

This anti-G-CSF antibody is a Rabbit Polyclonal antibody detecting G-CSF in WB and ELISA. Suitable for Human and Mouse. This Primary Antibody has been cited in 1 publication.

Quick Overview for G-CSF antibody (AA 47-208) (ABIN3042765)

Target: G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This G-CSF antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-G-CSF Antibody

Binding Specificity: AA 47-208

Purpose: Anti-G-CSF/CSF3 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-G-CSF/CSF3 Antibody Picoband® (ABIN3042765). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived mouse G-CSF recombinant protein (Position: R47-A208). Mouse G-CSF shares 77.2% amino acid (aa) sequence identity with human G-CSF.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse
ELISA, 0.1-0.5 μg/mL, -
1. Metcalf D (July 1985). "The granulocyte-macrophage colony-stimulating factors". Science 229 (4708): 16-22. 2. Nagata S, Tsuchiya M, Asano S, Kaziro Y, Yamazaki T, Yamamoto O, Hirata Y, Kubota N, Oheda M, Nomura H (1986). "Molecular cloning and expression of cDNA for human granulocyte colony-stimulating factor". Nature 319 (6052): 415-8. 3. Souza LM, Boone TC, Gabrilove J, Lai PH, Zsebo KM, Murdock DC, Chazin VR, Bruszewski J, Lu H, Chen KK, Barendt J, Platzer, E, Moore, MAS, Mertelsmann R, Welte K (April 1986). "Recombinant human granulocyte colony-stimulating factor: effects on normal and leukemic myeloid cells". Science 232 (4746): 61-5.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-G-CSF antibody (ABIN3042765)

Zhang, Wang, Wang, Jiang, Yu, Chong, Fu: "Ferulic acid ameliorates nerve injury induced by cerebral ischemia in rats." in: Experimental and therapeutic medicine, Vol. 9, Issue 3, pp. 972-976, (2015) (PubMed).

Target Details for G-CSF

Target: G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))

Alternative Name: Csf3

Background:

Synonyms: Granulocyte colony-stimulating factor,G-CSF,Csf3,Csfg,

Tissue Specificity: Expressed in all tissues examined including fetal brain, fibroblasts, heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon and leukocytes. Highest levels in heart and skeletal muscle. .

Background: Granulocyte-colony stimulating factor (G-CSF or GCSF), also known as colony-stimulating factor 3 (CSF 3), is a glycoprotein that stimulates the bone marrow to produce granulocytes and stem cells and release them into the bloodstream. Functionally, it is a cytokine and hormone, a type of colony-stimulating factor, and is produced by a number of different tissues. The pharmaceutical analogs of naturally occurring G-CSF are called filgrastim and lenograstim. G-CSF also stimulates the survival, proliferation, differentiation, and function of neutrophil precursors and mature neutrophils.

Sequence Similarities: Belongs to the DNA photolyase class-1 family.

Molecular Weight: 22 kDa

Gene ID: 12985

UniProt: P09920

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization