CXCR3 antibody (AA 1-368)

Catalog No. ABIN3042780

This anti-CXCR3 antibody is a Rabbit Polyclonal antibody detecting CXCR3 in WB and IHC. Suitable for Human and Rat. This Primary Antibody has been cited in 2+ publications.

Quick Overview for CXCR3 antibody (AA 1-368) (ABIN3042780)

Target: CXCR3 (Chemokine (C-X-C Motif) Receptor 3 (CXCR3))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CXCR3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-CXCR3 Antibody

Binding Specificity: AA 1-368

Purpose: Anti-CXCR3 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-CXCR3 Antibody Picoband® (ABIN3042780). Tested in IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CXCR3 recombinant protein (Position: M1-L368). Human CXCR3 shares 86% amino acid (aa) sequence identity with both mouse and rat CXCR3.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat
Western blot, 0.1-0.5 μg/mL, Human
1. Trentin, L., Agostini, C., Facco, M., Piazza, F., Perin, A., Siviero, M., Gurrieri, C., Galvan, S., Adami, F., Zambello, R., Semenzato, G. The chemokine receptor CXCR3 is expressed on malignant B cells and mediates chemotaxis. J. Clin. Invest. 104: 115-121, 1999. 2. Frigerio, S., Junt, T., Lu, B., Gerard, C., Zumsteg, U., Hollander, G. A., Piali, L. Beta cells are responsible for CXCR3-mediated T-cell infiltration in insulitis. Nature Med. 8: 1414-1420, 2002. 3. Feferman, T., Maiti, P. K., Berrih-Aknin, S., Bismuth, J., Bidault, J., Fuchs, S., Souroujon, M. C. Overexpression of IFN-induced protein 10 and its receptor CXCR3 in myasthenia gravis. J. Immun. 174: 5324-5331, 2005. 4. Struyf, S., Salogni, L., Burdick, M. D., Vandercappellen, J., Gouwy, M., Noppen, S., Proost, P., Opdenakker, G., Parmentier, M., Gerard, C., Sozzani, S., Strieter, R. M., Van Damme, J. Angiostatic and chemotactic activities of the CXC chemokine CXCL4L1 (platelet factor-4 variant) are mediated by CXCR3. Blood 117: 480-488, 2011.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-CXCR3 antibody (ABIN3042780)

Ding, Xia, Ding, Lu, Sun, Liu: "An alternatively spliced variant of CXCR3 mediates the metastasis of CD133+ liver cancer cells induced by CXCL9." in: Oncotarget, Vol. 7, Issue 12, pp. 14405-14, (2018) (PubMed).

Li, Zhu, Luo, Fang, Zhou, Hu, Maskey, Yang: "Impact of chemokine receptor CXCR3 on tumor-infiltrating lymphocyte recruitment associated with favorable prognosis in advanced gastric cancer." in: International journal of clinical and experimental pathology, Vol. 8, Issue 11, pp. 14725-32, (2016) (PubMed).

Target Details for CXCR3

Target: CXCR3 (Chemokine (C-X-C Motif) Receptor 3 (CXCR3))

Alternative Name: CXCR3

Background:

Synonyms: C-X-C chemokine receptor type 3,CXC-R3,CXCR-3,CKR-L2,G protein-coupled receptor 9,Interferon-inducible protein 10 receptor,IP-10 receptor,CD183,CXCR3,GPR9,

Tissue Specificity: Isoform 1 and isoform 2 are mainly expressed in heart, kidney, liver and skeletal muscle. Isoform 1 is also expressed in placenta. Isoform 2 is expressed in endothelial cells. Expressed in T-cells (at protein level). .

Background: Chemokine receptor CXCR3 is a Galphai protein-coupled receptor in the CXC chemokine receptor family. Other names for CXCR3 are G protein-coupled receptor 9 (GPR9) and CD183. It is mapped to Xq13.1. CXCR3 is expressed on malignant B cells from chronic lymphoproliferative disorders, particularly in patients with CLL, and represents a fully functional receptor involved in chemotaxis of malignant B lymphocytes. It is found that in the absence of known etiologic agents, CXCR3 represents a novel target for therapeutic interference early in type 1 diabetes. CXCR3 signaling is associated with MG pathogenesis and proposed that and CXCR3 may serve as novel drug targets to treat MG. CXCR3A and CXCR3B are involved in the chemotactic and vascular effects of CXCL4L1.

Sequence Similarities: Belongs to the G-protein coupled receptor 1 family.

Molecular Weight: 41 kDa

Gene ID: 2833

UniProt: P49682