CYLD antibody (AA 618-956)

Catalog No. ABIN3042782

The Rabbit Polyclonal anti-CYLD antibody has been validated for WB. It is suitable to detect CYLD in samples from Human and Rat.

Quick Overview for CYLD antibody (AA 618-956) (ABIN3042782)

Target: CYLD (Cylindromatosis (Turban Tumor Syndrome) (CYLD))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CYLD antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CYLD Antibody

Binding Specificity: AA 618-956

Purpose: Anti-CYLD Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-CYLD Antibody Picoband® (ABIN3042782). Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CYLD recombinant protein (Position: D618-K956). Human CYLD shares 97.6% and 97.9% amino acid (aa) sequence identity with mouse and rat CYLD, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Rat
1. Hayashi M, Jono H, Shinriki S, Nakamura T, Guo J, Sueta A, Tomiguchi M, Fujiwara S, Yamamoto-Ibusuki M, Murakami K, Yamashita S, Yamamoto Y, Li JD, Iwase H, Ando Y. ""Clinical significance of CYLD downregulation in breast cancer"". Breast Cancer Res Treat, 2014 Feb. 2. Vanecek T, Halbhuber Z, Kacerovska D, Martinek P, Sedivcova M, Carr RA, Slouka D, Michal M, Kazakov DV. ""Large germline deletions of the CYLD gene in patients with Brooke-Spiegler syndrome and multiple familial trichoepithelioma"". Am J Dermatopathol, 2014 Nov.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for CYLD

Target: CYLD (Cylindromatosis (Turban Tumor Syndrome) (CYLD))

Alternative Name: CYLD

Background:

Synonyms: Ubiquitin carboxyl-terminal hydrolase CYLD,3.4.19.12,Deubiquitinating enzyme CYLD,Ubiquitin thioesterase CYLD,Ubiquitin-specific-processing protease CYLD,CYLD,CYLD1, KIAA0849,HSPC057,

Tissue Specificity: Detected in fetal brain, testis, and skeletal muscle, and at a lower level in adult brain, leukocytes, liver, heart, kidney, spleen, ovary and lung. Isoform 2 is found in all tissues except kidney. .

Background: CYLD is localized on the long arm of chromosome 16. This gene encodes a cytoplasmic protein with three cytoskeletal-associated protein-glycine-conserved (CAP-GLY) domains that functions as a deubiquitinating enzyme. Mutations in this gene have been associated with cylindromatosis, multiple familial trichoepithelioma, and Brooke-Spiegler syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Sequence Similarities: Belongs to the peptidase C19 family.

Molecular Weight: 107 kDa

Gene ID: 1540

Pathways: Apoptosis, Activation of Innate immune Response