SP4 antibody (N-Term)

Catalog No. ABIN3043013

The Rabbit Polyclonal anti-SP4 antibody has been validated for WB. It is suitable to detect SP4 in samples from Human, Mouse and Rat.

Quick Overview for SP4 antibody (N-Term) (ABIN3043013)

Target: SP4 (Sp4 Transcription Factor (SP4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SP4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SP4 Antibody

Binding Specificity: AA 29-44, N-Term

Purpose: Anti-Transcription factor Sp4 Antibody Picoband®

Sequence: ENNNKKPKTS GSQDSQ

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Transcription factor Sp4 Antibody (ABIN3043013). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human SP4, identical to the related rat sequence, and different from the related mouse sequence by one amino acid.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Rat, Mouse
1. Hagen, G., Muller, S., Beato, M., Suske, G. Cloning by recognition site screening of two novel GT box binding proteins: a family of Sp1 related genes. Nucleic Acids Res. 20: 5519-5525, 1992. 2. Hagen, G., Dennig, J., Preiss, M., Beato, M., Suske, G.Functional analyses of the transcription factor Sp4 reveal properties distinct from Sp1 and Sp3. J. Biol. Chem. 270: 24989-24994, 1995.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for SP4

Target: SP4 (Sp4 Transcription Factor (SP4))

Alternative Name: SP4

Background:

Synonyms: Transcription factor Sp4;SPR-1;SP4;

Tissue Specificity: Abundant in brain.

Background: SP4 (Sp4 transcription factor), also known as Transcription factor Sp4, MGC130008, MGC130009, SPR-1, HF1B, is a protein that in humans is encoded by the SP4 gene. It is a multigene family of SP proteins. Northern blot analysis that SP4 is abundantly expressed as a 7.5-kb message in brain, but barely detectable in other human cell lines. SP4 is an activator protein like SP1. However, in contrast to SP1, SP4 is not able to act synergistically through adjacent binding sites.

Sequence Similarities: Belongs to the Sp1 C2H2-type zinc-finger protein family.

Molecular Weight: 82 kDa

UniProt: Q02446