TNNC1 antibody (N-Term)

Catalog No. ABIN3043055

The Rabbit Polyclonal anti-TNNC1 antibody has been validated for WB and IHC. It is suitable to detect TNNC1 in samples from Human, Mouse and Rat.

Quick Overview for TNNC1 antibody (N-Term) (ABIN3043055)

Target: TNNC1 (Cardiac Troponin C (TNNC1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TNNC1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-TNNC1 Antibody

Binding Specificity: AA 1-18, N-Term

Purpose: Anti-cardiac Troponin C/TNNC1 Antibody Picoband®

Sequence: MDDIYKAAVE QLTEEQKN

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-cardiac Troponin C/TNNC1 Antibody (ABIN3043055). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human cardiac Troponin C, identical to the related rat and mouse sequences .

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat, Mouse
Western blot, 0.1-0.5 μg/mL, Rat, Human, Mouse
1. Bermingham, N., Hernandez, D., Balfour, A., Gilmour, F., Martin, J. E., Fisher, E. M. C. Mapping TNNC1, the gene that encodes cardiac troponin I in the human and mouse. Genomics 30: 620-622, 1995. 2. Hoffmann, B., Schmidt-Traub, H., Perrot, A., Osterziel, K. J., Gessner, R. First mutation in cardiac troponin C, L29Q, in a patient with hypertrophic cardiomyopathy. Hum. Mutat. 17: 524 only, 2001. 3. Landstrom, A. P., Parvatiyar, M. S., Pinto, J. R., Marquardt, M. L., Bos, J. M., Tester, D. J., Ommen, S. R., Potter, J. D., Ackerman, M. J.Molecular and functional characterization of novel hypertrophic cardiomyopathy susceptibility mutations in TNNC1-encoded troponin C. J. Molec. Cell. Cardiol. 45: 281-288, 2008.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for TNNC1

Target: TNNC1 (Cardiac Troponin C (TNNC1))

Alternative Name: TNNC1

Background:

Synonyms: Troponin C, slow skeletal and cardiac muscles,TN-C,TNNC1,TNNC,

Background: Troponin C1 (TNNC1) (troponin C type 1 (slow)), also called TROPONIN C, SLOW-TWITCH SKELETAL MUSCLE or TNC, is a protein that in humans is encoded by the TNNC1 gene. Using a "monochromosomal" hybrid panel, the TNNC1 gene is mapped to chromosome 3, subsequent analysis of the Genebridge 4 radiation hybrid panel localized the gene to 3p in a region consistent with the earlier assignment. Circular dichroism measurements revealed changes in the secondary structures of the TNNC1 mutants A8V, C84Y, and D145E.

Sequence Similarities: Belongs to the troponin C family.

Molecular Weight: 18 kDa

UniProt: P63316