Monoamine Oxidase A antibody (N-Term)

Catalog No. ABIN3043133

The Rabbit Polyclonal anti-Monoamine Oxidase A antibody has been validated for WB and IHC. It is suitable to detect Monoamine Oxidase A in samples from Human, Mouse and Rat.

Quick Overview for Monoamine Oxidase A antibody (N-Term) (ABIN3043133)

Target: Monoamine Oxidase A (MAOA)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Monoamine Oxidase A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Monoamine Oxidase A Antibody

Binding Specificity: AA 51-69, N-Term

Purpose: Anti-Monoamine Oxidase A/MAOA Antibody Picoband®

Sequence: RTYTIRNEHV DYVDVGGAY

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Monoamine Oxidase A/MAOA Antibody (ABIN3043133). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human MAOA.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. Breakefield, X. O., Ozelius, L., Hsu, Y. P., Powell, J., Utterback, M., Gusella, J. F., Bruns, G. A. Gene for A form of human monoamine oxidase (MAOA) maps to Xp21-Xp11. (Abstract) Am. J. Hum. Genet. 41: A209 only, 1987. 2. Brunner, H. G., Nelen, M., Breakefield, X. O., Ropers, H. H., van Oost, B. A. Abnormal behavior associated with a point mutation in the structural gene for monoamine oxidase A. Science 262: 578-580, 1993. 3. PDB 2BXS, De Colibus L, Li M, Binda C, Lustig A, Edmondson DE, Mattevi A (September 2005). "Three-dimensional structure of human monoamine oxidase A (MAO A): relation to the structures of rat MAO A and human MAO B".

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Monoamine Oxidase A

Target: Monoamine Oxidase A (MAOA)

Alternative Name: MAOA

Background:

Synonyms: Amine oxidase [flavin-containing] A,1.4.3.4,Monoamine oxidase type A,MAO-A,MAOA,

Tissue Specificity: Heart, liver, duodenum, blood vessels and kidney.

Background: MAOA (Monoamine oxidase A), also known as AMINE OXIDASE (FLAVIN-CONTAINING) A, is an enzyme that in humans is encoded by the MAO-A gene. MAOA is an isozyme of monoamine oxidase which is also mapped on Xp11.3. MAOA degrades amine neurotransmitters, such as dopamine, norepinephrine, and serotonin. The protein localizes to the outer mitochondrial membrane. Mutation in MAOA results in monoamine oxidase deficiency, or Brunner syndrome. In humans, there is a 30-base repeat sequence repeated in one of several different numbers of times in the promoter region of the gene coding for MAOA. MAO-A levels in the brain as measured using positron emission tomography are elevated by an average of 34 % in patients with major depressive disorder. Inhibition of MAOA prevented apoptosis, and serum starvation of cortical brain cells from Maoa-deficient mice resulted in reduced apoptosis compared with wildtype mice.

Sequence Similarities: Belongs to the flavin monoamine oxidase family.

Molecular Weight: 65 kDa

UniProt: P21397