Myeloperoxidase antibody (C-Term)

Catalog No. ABIN3043232

The Rabbit Polyclonal anti-Myeloperoxidase antibody has been validated for WB, IHC, FACS and IF. It is suitable to detect Myeloperoxidase in samples from Human, Mouse and Rat. There are 3+ publications available.

Quick Overview for Myeloperoxidase antibody (C-Term) (ABIN3043232)

Target: Myeloperoxidase (MPO)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Myeloperoxidase antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Product Details anti-Myeloperoxidase Antibody

Binding Specificity: AA 714-728, C-Term

Purpose: Anti-Myeloperoxidase/MPO Antibody Picoband®

Sequence: KNNIFMSNSY PRDFV

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Myeloperoxidase/MPO Antibody (ABIN3043232). Tested in Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human MPO, different from the related mouse and rat sequences by one amino acid.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Klebanoff, S. J. : Myeloperoxidase. Proc. Assoc. Am. Phys. 111: 383-389, 1999. 2. Murao, S.-I., Stevens, F. J., Ito, A., Huberman, E. : Myeloperoxidase: a myeloid cell nuclear antigen with DNA-binding properties. Proc. Nat. Acad. Sci. 85: 1232-1236, 1988. 3. Nauseef, W. M., Olsson, I., Arnljots, K. : Biosynthesis and processing of myeloperoxidase--a marker for myeloid cell differentiation. Europ. J. Haemat. 40: 97-110, 1988.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-Myeloperoxidase antibody (ABIN3043232)

Wei, Kong, Li, Guan, Wang, Miao: "Nicotinamide mononucleotide attenuates brain injury after intracerebral hemorrhage by activating Nrf2/HO-1 signaling pathway." in: Scientific reports, Vol. 7, Issue 1, pp. 717, (2018) (PubMed).

Xu, Zhang, He, Li, Gao, Liu: "Lactobacillus acidophilus alleviates pouchitis after ileal pouch-anal anastomosis in rats." in: World journal of gastroenterology, Vol. 23, Issue 26, pp. 4735-4743, (2018) (PubMed).

Yu, Chen, Wang, Kuang, Liu, Zhang, Du: "Neuroprotective effect of kaempferol glycosides against brain injury and neuroinflammation by inhibiting the activation of NF-?B and STAT3 in transient focal stroke." in: PLoS ONE, Vol. 8, Issue 2, pp. e55839, (2013) (PubMed).

Target Details for Myeloperoxidase

Target: Myeloperoxidase (MPO)

Alternative Name: MPO

Background:

Synonyms: Myeloperoxidase,MPO,1.11.2.2,Myeloperoxidase,89 kDa myeloperoxidase,84 kDa myeloperoxidase,Myeloperoxidase light chain,Myeloperoxidase heavy chain,MPO,

Tissue Specificity: MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system. .

Background: Myeloperoxidase (MPO) is a mammalian phagocyte hemoprotein though to primarily mediate host defense reactions. It is abundantly expressed in neutrophils and secreted during their activation. Myeloperoxidase is part of the host defense system of human polymorphonuclear leukocytes, responsible for microbicidal activity against a wide range of organisms. It is located in the nucleus as well as in the cytoplasm. Intranuclear MPO may help to protect DNA against damage resulting from oxygen radicals produced during myeloid cell maturation and function.

Sequence Similarities: Belongs to the peroxidase family. XPO subfamily.

Molecular Weight: 60 kDa

UniProt: P05164

Pathways: Chromatin Binding