KNG1 antibody (Middle Region)

Catalog No. ABIN3043285

This Rabbit Polyclonal antibody specifically detects KNG1 in WB and IHC. It exhibits reactivity toward Mouse.

Quick Overview for KNG1 antibody (Middle Region) (ABIN3043285)

Target: KNG1 (Kininogen 1 (KNG1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KNG1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-KNG1 Antibody

Binding Specificity: AA 227-259, Middle Region

Purpose: Anti-Kininogen 1/KNG1 Antibody Picoband®

Sequence: ECRGNLFMDI NNKIANFSQS CTLYSGDDLV EA

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Kininogen 1/KNG1 Antibody Picoband® (ABIN3043285). Tested in IHC, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of mouse Kininogen 1, different from the related rat sequence by thirteen amino acids.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Mouse
Western blot, 0.1-0.5 μg/mL, Mouse
1. "Entrez Gene: kininogen 1". 2. Fong, D., Smith, D. I., Hsieh, W.-T. The human kininogen gene (KNG) mapped to chromosome 3q26-qter by analysis of somatic cell hybrids using the polymerase chain reaction. Hum. Genet. 87: 189-192, 1991.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for KNG1

Target: KNG1 (Kininogen 1 (KNG1))

Alternative Name: Kng1

Background:

Synonyms: Kininogen-1,Kininogen-1 heavy chain,Bradykinin,Kininogen-1 light chain,Kng1,Kng,

Tissue Specificity: Plasma.

Background: Kininogen-1 (KNG1), also known as BDK or bradykinin, is a protein that in humans is encoded by the KNG1 gene. It is mapped to 3q27.3. The KNG1 gene uses alternative splicing to generate two different proteins - high - molecular - weight kininogen (HMWK) and low - molecular- weight kininogen (LMWK). HMWK is essential for blood coagulation and assembly of the kallikrein-kinin system. Also, KNG1, a peptide causing numerous physiological effects, is released from HMWK. In contrast to HMWK, LMWK is not involved in blood coagulation. In addition to that, KNG1 is a constituent of the blood coagulation system as well as the kinin-kallikrein system.

Sequence Similarities: Contains 1 HMG box DNA-binding domain.

Molecular Weight: 73 kDa

Gene ID: 16644

UniProt: O08677

Pathways: ACE Inhibitor Pathway, Glycosaminoglycan Metabolic Process