SEMA3A antibody (AA 550-771)

Catalog No. ABIN3043328

Product Details anti-SEMA3A Antibody

Target: SEMA3A (Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3A (SEMA3A))

Binding Specificity: AA 550-771

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SEMA3A antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Anti-Semaphorin 3A/SEMA3A Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Semaphorin 3A/SEMA3A Antibody Picoband® (ABIN3043328). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Semaphorin 3A recombinant protein (Position: K550-V771). Human Semaphorin 3A shares 93% and 94% amino acid (aa) sequence identity with mouse and rat Semaphorin 3A, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
1. Nakano Y, et al. A nonsynonymous polymorphism in semaphorin 3A as a risk factor for human unexplained cardiac arrest with documented ventricular fibrillation. PLoS Genet, 2013 Apr. 2. Takagawa S, et al. Decreased semaphorin3A expression correlates with disease activity and histological features of rheumatoid arthritis. BMC Musculoskelet Disord, 2013 Jan 23. 3. Vadasz Z, et al. The role of B regulatory cells and Semaphorin3A in atopic diseases. Int Arch Allergy Immunol, 2014.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-SEMA3A antibody (ABIN3043328)

Zhou, Wu, Fu, Lv, Zhang: "Significance of semaphorin-3A and MMP-14 protein expression in non-small cell lung cancer." in: Oncology letters, Vol. 7, Issue 5, pp. 1395-1400, (2014) (PubMed).

Target Details for SEMA3A

Target: SEMA3A (Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3A (SEMA3A))

Alternative Name: SEMA3A (SEMA3A Products)

Background:

Synonyms: Semaphorin-3A,Semaphorin III,Sema III,SEMA3A,SEMAD,

Background: SEMA3A is also known as COLL1, SEMA1 or SEMAL. This gene is a member of the semaphorin family and encodes a protein with an Ig-like C2-type (immunoglobulin-like) domain, a PSI domain and a Sema domain. This secreted protein can function as either a chemorepulsive agent, inhibiting axonal outgrowth, or as a chemoattractive agent, stimulating the growth of apical dendrites. In both cases, the protein is vital for normal neuronal pattern development. Increased expression of this protein is associated with schizophrenia and is seen in a variety of human tumor cell lines. Also, aberrant release of this protein is associated with the progression of Alzheimer's disease.

Sequence Similarities: Belongs to the semaphorin family.

Molecular Weight: 89 kDa

Gene ID: 10371

UniProt: Q14563

Pathways: Regulation of Cell Size