Sp2 antibody (Middle Region)

Catalog No. ABIN3043349

The Rabbit Polyclonal anti-Sp2 antibody has been validated for WB. It is suitable to detect Sp2 in samples from Human and Rat.

Quick Overview for Sp2 antibody (Middle Region) (ABIN3043349)

Target: Sp2 (Sp2 Transcription Factor (Sp2))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Sp2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Sp2 Antibody

Binding Specificity: AA 312-343, Middle Region

Purpose: Anti-SP2 transcription factor Antibody Picoband®

Sequence: QVVQIPQQAL RVVQAASATL PTVPQKPSQN FQ

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-SP2 transcription factor Antibody Picoband® (ABIN3043349). Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human SP2, identical to the related mouse sequence.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Rat
1. Kingsley C, Winoto A (Oct 1992). "Cloning of GT box-binding proteins: a novel Sp1 multigene family regulating T-cell receptor gene expression". Mol Cell Biol 12 (10): 4251-61. 2. Rotheneder H, Geymayer S, Haidweger E (Nov 1999). "Transcription factors of the Sp1 family: interaction with E2F and regulation of the murine thymidine kinase promoter". J. Mol. Biol. 293 (5): 1005-15. 3. Scohy S, Van Vooren P, Szpirer C, Szpirer J (Oct 1998). "Assignment1 of Sp genes to rat chromosome bands 7q36 (Sp1), 10q31→q32.1 (Sp2), 3q24→q31 (Sp3) and 6q33 (Sp4) and of the SP2 gene to human chromosome bands 17q21.3→q22 by in situ hybridization". Cytogenet Cell Genet 81 (3-4): 273-4.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for Sp2

Target: Sp2 (Sp2 Transcription Factor (Sp2))

Alternative Name: SP2

Background:

Synonyms: Transcription factor Sp2,SP2,KIAA0048,

Tissue Specificity: Most abundant in lung, less in heart and skin. .

Background: Transcription factor Sp2 is a protein that in humans is encoded by the SP2 gene. This gene encodes a member of the Sp subfamily of Sp/XKLF transcription factors. Sp family proteins are sequence-specific DNA-binding proteins characterized by an amino-terminal trans-activation domain and three carboxy-terminal zinc finger motifs. This protein contains the least conserved DNA-binding domain within the Sp subfamily of proteins, and its DNA sequence specificity differs from the other Sp proteins. It localizes primarily within subnuclear foci associated with the nuclear matrix, and can activate or in some cases repress expression from different promoters.

Sequence Similarities: Belongs to the Sp1 C2H2-type zinc-finger protein family.

Molecular Weight: 72 kDa

Gene ID: 6668

UniProt: Q02086