CD63 antibody (AA 97-238)

Catalog No. ABIN3043395

This anti-CD63 antibody is a Rabbit Polyclonal antibody detecting CD63 in WB and IHC. Suitable for Human. This Primary Antibody has been cited in 2+ publications.

Quick Overview for CD63 antibody (AA 97-238) (ABIN3043395)

Target: CD63

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD63 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-CD63 Antibody

Binding Specificity: AA 97-238

Purpose: Anti-CD63 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-CD63 Antibody Picoband® (ABIN3043395). Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CD63 recombinant protein (Position: E97-M238). Human CD63 shares 74% and 73% amino acid (aa) sequence identity with mouse and rat CD63, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
1. "Entrez Gene: CD63 CD63 Molecule" 2. Gwynn, B., Eicher, E. M., Peters, L. L. Genetic localization of Cd63, a member of the transmembrane 4 superfamily, reveals two distinct loci in the mouse genome. Genomics 35: 389-391, 1996.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-CD63 antibody (ABIN3043395)

Ren, Wang, Yuan, Wang: "Dendritic cells loaded with HeLa-derived exosomes simulate an antitumor immune response." in: Oncology letters, Vol. 15, Issue 5, pp. 6636-6640, (2018) (PubMed).

Du, Dong, Zhao, Fu, Wang, Chen, Ou, Li, Sun, Tang, Song: "Immunostimulatory and anti-neoplasm effects of a novel palindrome CpG oligodeoxynucleotide in mice." in: Acta pharmacologica Sinica, Vol. 33, Issue 8, pp. 1047-54, (2012) (PubMed).

Target Details for CD63

Target: CD63

Alternative Name: CD63

Background:

Synonyms: CD63 antigen,Granulophysin,Lysosomal-associated membrane protein 3,LAMP-3,Melanoma-associated antigen ME491,OMA81H,Ocular melanoma-associated antigen,Tetraspanin-30,Tspan-30,CD63,CD63,MLA1, TSPAN30,

Tissue Specificity: Detected in platelets (at protein level). Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages. .

Background: CD63 antigen is a protein that in humans is encoded by the CD63 gene. The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. It is mapped to 12q13.2. CD63 is mainly associated with membranes of intracellular vesicles, although cell surface expression may be induced. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression.

Molecular Weight: 30-60 kDa

Gene ID: 967

UniProt: P08962