SHBG antibody (AA 30-210)

Catalog No. ABIN3043434

This anti-SHBG antibody is a Rabbit Polyclonal antibody detecting SHBG in WB. Suitable for Human.

Quick Overview for SHBG antibody (AA 30-210) (ABIN3043434)

Target: SHBG (Sex Hormone Binding Globulin (SHBG))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SHBG antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SHBG Antibody

Binding Specificity: AA 30-210

Purpose: Anti-Sex Hormone Binding Globulin/SHBG Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Sex Hormone Binding Globulin/SHBG Antibody Picoband® (ABIN3043434). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human SHBG recombinant protein (Position: L30-A210). Human SHBG shares 72% amino acid (aa) sequence identity with both mouse and rat SHBG.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
1. Hammond GL (2011). "Diverse Roles for Sex Hormone-Binding Globulin in Reproduction". Biology of Reproduction 85(3): 431-441. 2. Selva, D. M., Hogeveen, K. N., Innis, S. M., Hammond, G. L. Monosaccharide-induced lipogenesis regulates the human hepatic sex hormone-binding globulin gene. J. Clin. Invest. 117: 3979-3987, 2007. 3. Simó R, Barbosa-Desongles A, Hernandez C, Selva DM (November 2012). "IL1β down-regulation of sex hormone-binding globulin production by decreasing HNF-4α via MEK-1/2 and JNK MAPK pathways". Mol. Endocrinol. 26 (11): 1917-27.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for SHBG

Target: SHBG (Sex Hormone Binding Globulin (SHBG))

Alternative Name: SHBG

Background:

Synonyms: Sex hormone-binding globulin,SHBG,Sex steroid-binding protein,SBP,Testis-specific androgen-binding protein,ABP,Testosterone-estradiol-binding globulin,TeBG,Testosterone-estrogen-binding globulin,SHBG,

Tissue Specificity: Isoform 1 and isoform 2 are present in liver and testis.

Background: Sex hormone-binding globulin (SHBG) is a glycoprotein that binds to the sex hormones,androgen and estrogen. SHBG is produced mostly by the liver and is released into the bloodstream. Other sites that produce SHBG include the brain, uterus, testes, and placenta. SHBG has both enhancing and inhibiting hormonal influences. It decreases with high levels of insulin, growth hormone, insulin-like growth factor 1 (IGF-1), androgens, prolactin and transcortin. High estrogen, andthyroxine cause it to increase. It has been found that the monosaccharides glucose and fructose reduced human SHBG production by hepatocytes, irrespective of the presence of insulin, by downregulation of hepatocyte nuclear factor-4A and replacement of HNF4A by COUP-TFI at a cis-element within the human SHBG promoter, coincident with repression of its transcriptional activity.

Sequence Similarities: Contains 2 laminin G-like domains.

Molecular Weight: 70 kDa

Gene ID: 6462

UniProt: P04278

Pathways: Hormone Transport