MUC5AC antibody (AA 4848-5030)

Catalog No. ABIN3043460

The Rabbit Polyclonal anti-MUC5AC antibody has been validated for IHC. It is suitable to detect MUC5AC in samples from Human and Rat. There is 1 publication available.

Quick Overview for MUC5AC antibody (AA 4848-5030) (ABIN3043460)

Target: MUC5AC (Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MUC5AC antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Product Details anti-MUC5AC Antibody

Binding Specificity: AA 4848-5030

Purpose: Anti-Mucin 5AC/MUC5AC Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Mucin 5AC/MUC5AC Antibody Picoband® (ABIN3043460). Tested in IHC applications. This antibody reacts with Human, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Mucin 5AC recombinant protein (Position: D4848-H5030).

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Rat
1. Hasnain, S. Z., Evans, C. M., Roy, M., Gallagher, A. L., Kindrachuk, K. N., Barron, L., Dickey, B. F., Wilson, M. S., Wynn, T. A., Grencis, R. K., Thornton, D. J. Muc5ac: a critical component mediating the rejection of enteric nematodes. J. Exp. Med. 208: 893-900, 2011. 2. Moehle, C., Ackermann, N., Langmann, T., Aslanidis, C., Kel, A., Kel-Margoulis, O., Schmitz-Madry, A., Zahn, A., Stremmel, W., Schmitz, G. Aberrant intestinal expression and allelic variants of mucin genes associated with inflammatory bowel disease. J. Molec. Med. 84: 1055-1066, 2006. 3. Wang G, Xu Z, Wang R, Al-Hijji M, Salit J, Strulovici-Barel Y, Tilley A, Mezey J, Crystal R (June 2012). "Genes associated with MUC5AC expression in small airway epithelium of human smokers and nonsmokers". BMC Med Genomics5 (1): 21.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-MUC5AC antibody (ABIN3043460)

Zhou, Perelman, Kolosov, Zhou: "Neutrophil elastase induces MUC5AC secretion via protease-activated receptor 2." in: Molecular and cellular biochemistry, Vol. 377, Issue 1-2, pp. 75-85, (2013) (PubMed).

Target Details for MUC5AC

Target: MUC5AC (Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC))

Alternative Name: MUC5AC

Background:

Synonyms: Mucin-5AC ,MUC-5AC ,Gastric mucin ,Lewis B blood group antigen ,LeB ,Major airway glycoprotein ,Mucin-5 subtype AC, tracheobronchial,Tracheobronchial mucin ,TBM ,MUC5AC ,MUC5 ,

Tissue Specificity: Highly expressed in surface mucosal cells of respiratory tract and stomach epithelia. Overexpressed in a number of carcinomas. Also expressed in Barrett's esophagus epithelium and in the proximal duodenum. .

Background: Mucin-5AC is a protein that in humans is encoded by the MUC5AC gene. It is mapped to 11p15.5. MUC5AC is highly expressed in adult trachea and stomach. This gene has been linked to mucus hypersecretion in the pulmonary tracts and associated to chronic obstructive pulmonary disease (COPD). It has been showed that activation of the NF-kappa-B signaling pathway by inflammatory cytokine TNF-alpha upregulated MUC5AC mRNA expression nearly 6-fold. MUC5AC is also a and critical mediator of resistance during intestinal nematode infection.

Sequence Similarities: Contains 1 CTCK (C-terminal cystine knot-like) domain.

Molecular Weight: 585 kDa

Gene ID: 4586

UniProt: P98088